Peng Wei, Hu Yujuan, Zhong Yi, Chen Bei, Sun Yu, Yang Yang, Kong Weijia
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2010 Aug;30(4):514-8. doi: 10.1007/s11596-010-0460-2. Epub 2010 Aug 17.
The protective roles of alpha-lipoic acid in the rat model of mitochondrial DNA (mtDNA) 4834bp deletion in inner ear were investigated. Forty female Wistar rats at 4 weeks of age were divided into four groups: group A (D-galactose group, n=10), group B (D-galactose+alpha-lipoic acid group, n=10), group C (alpha-lipoic acid group, n=10), and group D (control group, n=10). Auditory brainstem response (ABR) was used to detect the hearing threshold. Colorimetry was used to analyze activity of superoxide dismutase (SOD) and concentration of malondialdehyde (MDA). The percentage of mtDNA4834bp deletion in inner ear was identified by real-time PCR. There was no significant difference in ABR threshold shift among all groups. The percentage of mtDNA4834bp deletion in group A was higher than that in other groups, but there was no significant difference in percentage of mtDNA4834bp deletion among groups B, C, and D. The activity of SOD in group A was lower than that in other groups. The concentration of MDA in group A was higher than that in other groups. It was concluded that there was no significant hearing loss when the percentage of mtDNA4834bp deletion was lower than 12.5%. Alpha-lipoic acid could prevent the reactive oxygen species (ROS)-induced mtDNA4834bp deletion in inner ear of rats.
研究了α-硫辛酸在大鼠内耳线粒体DNA（mtDNA）4834bp缺失模型中的保护作用。将40只4周龄雌性Wistar大鼠分为四组：A组（D-半乳糖组，n = 10）、B组（D-半乳糖+α-硫辛酸组，n = 10）、C组（α-硫辛酸组，n = 10）和D组（对照组，n = 10）。采用听性脑干反应（ABR）检测听阈。采用比色法分析超氧化物歧化酶（SOD）活性和丙二醛（MDA）浓度。通过实时荧光定量PCR鉴定内耳mtDNA4834bp缺失的百分比。所有组间ABR阈值变化无显著差异。A组mtDNA4834bp缺失百分比高于其他组，但B、C、D组间mtDNA4834bp缺失百分比无显著差异。A组SOD活性低于其他组。A组MDA浓度高于其他组。得出结论：当mtDNA4834bp缺失百分比低于12.5%时，无明显听力损失。α-硫辛酸可预防活性氧（ROS）诱导的大鼠内耳mtDNA4834bp缺失。
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