Département de Chimie, Université de Montréal, CP6128, Succursale Centre-Ville, Montréal, Québec H3C 3J7, Canada.
J Microencapsul. 2010;27(8):703-13. doi: 10.3109/02652048.2010.509518. Epub 2010 Aug 17.
Microcapsules for enzyme immobilization were successfully fabricated via interfacial cross-linking of poly(ethyleneimine) (PEI). A method based on laminar jet break-up technique using a commercial instrument developed to produce alginate beads is reported for the first time for production of PEI microcapsules. The diameter, wall thickness and pore size of membranes were obtained from confocal laser scanning microscopy by labelling PEI and proteins. The composition of membranes was analysed by elemental analysis. Larger microcapsules (ca 200 µm diameter) were obtained with the encapsulation device. In comparison, the emulsion method produced smaller capsules (ca 20 µm diameter) but with a wider size distribution. Encapsulation efficiency for both methods was analysed by bicinchoninic acid and fluorescence assays, yielding efficiencies of 94 ± 2% and 83 ± 3% for the emulsion method and encapsulation device, respectively. Glucose oxidase from Aspergillus Niger and Laccase from Trametes Versicolor were encapsulated by both microencapsulation methods and their activities were compared.
通过聚（亚乙基亚胺）（PEI）的界面交联成功制备了用于酶固定化的微胶囊。首次报道了一种基于层流射流破裂技术的方法，使用商业仪器生产海藻酸盐珠，用于生产 PEI 微胶囊。通过标记 PEI 和蛋白质，从共聚焦激光扫描显微镜获得膜的直径、壁厚和孔径。通过元素分析分析膜的组成。通过封装装置获得了更大的微胶囊（约 200 µm 直径）。相比之下，乳液法产生了更小的胶囊（约 20 µm 直径），但尺寸分布更宽。通过双缩脲和荧光测定法分析了两种方法的包封效率，乳液法和封装装置的效率分别为 94±2%和 83±3%。黑曲霉葡萄糖氧化酶和变色栓菌漆酶均通过两种微胶囊化方法进行包封，并比较了它们的活性。
