Institut für Klinische Pathologie, UniversitätsSpital Zürich, Schmelzbergstrasse 12, Zürich, Switzerland.
Histopathology. 2010 Aug;57(2):244-9. doi: 10.1111/j.1365-2559.2010.03618.x.
As tumour specimens and biopsy specimens become smaller, recognition of anatomical structures relevant for staging is increasingly challenging. So far no marker is known that reliably discriminates between muscularis propria (MP) and muscularis mucosae (MM) of the gastrointestinal tract. Recently, smoothelin expression has been shown to differ in MP and MM of the urinary bladder. We aimed to analyse the expression of smoothelin in the gastrointestinal tract in MP and MM in order to define a novel diagnostic tool to identify MM bundles.
The expression of smoothelin was analysed immunohistochemically in comparison with alpha-smooth muscle actin (alpha-SMA) in specimens from colon, stomach and oesophagus (n = 107). In contrast to alpha-SMA, which equally stained MM and MP, absent or significantly weaker smoothelin expression was found in MM was found, which was particularly valid in colon and gastric specimens.
The combination of smoothelin and SMA represents a robust marker to discriminate MM from MP in the gastrointestinal tract.
随着肿瘤标本和活检标本的体积变小，对于与分期相关的解剖结构的识别变得越来越具有挑战性。到目前为止，还没有已知的标志物能够可靠地区分胃肠道的固有肌层（MP）和黏膜肌层（MM）。最近，平滑肌蛋白在膀胱的 MP 和 MM 中的表达已经显示出不同。我们旨在分析 smoothelin 在胃肠道的 MP 和 MM 中的表达，以定义一种新的诊断工具来识别 MM 束。
我们通过免疫组织化学分析 smoothelin 的表达，并与结肠、胃和食管的标本中的α-平滑肌肌动蛋白（alpha-SMA）进行比较（n=107）。与 alpha-SMA 不同，它均匀地染色 MM 和 MP，在 MM 中发现 smoothelin 的表达缺失或明显减弱，这在结肠和胃标本中尤其有效。
在胃肠道中，smoothelin 和 SMA 的组合是区分 MM 和 MP 的可靠标志物。
