Research Laboratories for Fundamental Technology of Food, Asahi Breweries, Ltd., Ibaraki, Japan.
Planta Med. 2011 Jan;77(2):122-7. doi: 10.1055/s-0030-1250204. Epub 2010 Aug 17.
Apple polyphenols (AP) mainly consist of procyanidins (PC), which are composed of (-)-epicatechins and (+)-catechins. In order to investigate the antiageing effects of PC, we measured the lifespan of CAENORHABDITIS ELEGANS worms treated with PC. Treatment with 65 µg/mL PC extended the mean lifespan of wild-type N2 and FEM-1 worms by 12.1 % and 8.4 %, respectively, i.e., to a similar extent as resveratrol. In addition, treatment with 100 µg/mL AP also significantly prolonged the mean lifespan of the same worms by 12.0 % and 5.3 %, respectively, i.e., to a similar extent as PC. In contrast, treatment with (-)-epicatechin did not extend the lifespan of the worms. PC did not modify the growth, food intake, or fecundity of C. elegans. Treatment with PC did not extend the lifespan of MEV-1 worms, which show excessive oxidative stress, indicating that PC had no antioxidant ability in the MEV-1 mutant. Moreover, treatment with PC had no effect on the longevity of SIR-2.1 worms, which lack the activity of SIR-2, a member of the sirtuin family of NAD (+)-dependent protein deacetylases. These results indicated that PC has SIR-2.1-dependent antiageing effects on C. elegans.
苹果多酚（AP）主要由原花青素（PC）组成，原花青素由（-）-表儿茶素和（+）-儿茶素组成。为了研究 PC 的抗衰老作用，我们测量了用 PC 处理的秀丽隐杆线虫的寿命。用 65µg/mL PC 处理，野生型 N2 和 FEM-1 线虫的平均寿命分别延长了 12.1%和 8.4%，与白藜芦醇的效果相当。此外，用 100µg/mL AP 处理也分别显著延长了相同线虫的平均寿命 12.0%和 5.3%，与 PC 的效果相当。相比之下，（-）-表儿茶素处理并没有延长线虫的寿命。PC 不改变秀丽隐杆线虫的生长、食物摄入或繁殖力。PC 处理并没有延长 MEV-1 线虫的寿命，MEV-1 线虫表现出过度的氧化应激，表明 PC 在 MEV-1 突变体中没有抗氧化能力。此外，PC 处理对缺乏 SIR-2 活性的 SIR-2.1 线虫的寿命没有影响，SIR-2 是 NAD（+）依赖性蛋白去乙酰酶家族中 Sirtuin 的一个成员。这些结果表明，PC 对线虫具有 SIR-2.1 依赖性的抗衰老作用。
