[hsa-miR-125a-5p增强非小细胞肺癌细胞系的侵袭能力。]
[hsa-miR-125a-5p Enhances Invasion Ability in Non-Small Lung Carcinoma Cell Lines.].
作者信息
Jiang Lili, Zhang Qingfu, Chang Jihong, Qiu Xueshan, Wang Enhua
机构信息
Department of Pathology, College of Basic Medical Sciences, China Medical University, and Department of Pathology, the First Hospital of China Medical University, Shenyang 110001, China.
出版信息
Zhongguo Fei Ai Za Zhi. 2009 Sep 20;12(9):951-5. doi: 10.3779/j.issn.1009-3419.2009.09.002.
BACKGROUND
MicroRNAs (miRNAs) are short non-coding RNAs that posttranscriptionally regulate gene expression by partially binding complementary to target sites in mRNAs. Although some impaired miRNA regulations have been observed in many human cancers, the functions of miR-125a are still unclear. The aim of this study is to investigate the expression of hsa-miR-125a-5p in NSCLC cell lines and the relationship between hsa-miR-125a-5p and the invasion of lung cancer cells.
METHODS
The expression of hsa-miR-125a-5p and the effectiveness for a given period time after being transfected sense hsa-miR-125a-5p 2'-O-methyl oligonucleotide, which were 24 h, 36 h, 48 h, 60 h and 72 h, were examined by realtime PCR. Meanwhile, we investigated the modification of invasive ability in A549 and NCI-H460 cells by transwell.
RESULTS
Real-time PCR showed that hsa-miR-125a-5p was poorly-expressed in 6 lung cancer cell lines, especially in LH7, NCI-H460, SPC-A-1 and A549. The highest expression of hsa-miR-125a-5p occurred in the cells transfected with sense hsa-miR-125a-5p 2'-O-methyl oligonucleotide 36 h. Furthermore, the invasive abilities of A549 and NCI-H46O were enhanced by up-regulating hsa-miR-125a-5p.
CONCLUSIONS
hsa-miR-125a-5p was poorly-expressed in lung cancer cells and it could enhance lung cancer cell invasion by up-regulating hsa-miR-125a-5p.
背景
微小RNA（miRNA）是短链非编码RNA，通过与mRNA上的靶位点部分互补结合，在转录后水平调控基因表达。尽管在许多人类癌症中已观察到一些miRNA调控受损，但miR-125a的功能仍不清楚。本研究旨在探讨hsa-miR-125a-5p在非小细胞肺癌（NSCLC）细胞系中的表达及其与肺癌细胞侵袭的关系。
方法
通过实时PCR检测hsa-miR-125a-5p的表达以及转染正义hsa-miR-125a-5p 2'-O-甲基寡核苷酸后24小时、36小时、48小时、60小时和72小时这一给定时间段内的效果。同时，我们通过Transwell实验研究A549和NCI-H460细胞侵袭能力的变化。
结果
实时PCR显示，hsa-miR-125a-5p在6种肺癌细胞系中表达较低，尤其是在LH7、NCI-H460、SPC-A-1和A549细胞系中。hsa-miR-125a-5p在转染正义hsa-miR-125a-5p 2'-O-甲基寡核苷酸36小时后的细胞中表达最高。此外，上调hsa-miR-125a-5p可增强A549和NCI-H46O细胞的侵袭能力。
结论
hsa-miR-125a-5p在肺癌细胞中表达较低，上调hsa-miR-125a-5p可增强肺癌细胞的侵袭能力。
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