Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Biosens Bioelectron. 2010 Oct 15;26(2):463-9. doi: 10.1016/j.bios.2010.07.084. Epub 2010 Jul 30.
A simple and reliable method based on voltammetry is proposed for the determination of oxidative DNA damage by the simultaneous determination of guanine and 8-hydroxygunine using single-walled carbon nanotubes modified edge plane pyrolytic graphite electrode (SWNT/EPPGE). In acid-hydrolyzed calf thymus DNA, two well-defined peaks at ∼312 and ∼502 mV corresponding to the oxidation of 8-hydroxyguanine and guanine, respectively in phosphate buffer solution (PBS) of pH 7.2 were observed in square wave voltammetry. Remarkable enhancement in the oxidation peak current of both the compounds was observed along with the negative shift of peak potentials using SWNT/EPPGE as compared to bare EPPGE. The detection limits of guanine and 8-hydroxyguanine were calculated to be 0.05×10(-9) and 0.01×10(-9) mol L(-1), respectively. The limits of quantification were found as 0.17×10(-9) and 0.34×10(-10) mol L(-1) for guanine and 8-hydroxyguanine, respectively.
提出了一种基于伏安法的简单可靠的方法，用于通过使用单壁碳纳米管修饰的边缘平面热解石墨电极（SWNT / EPPGE）同时测定鸟嘌呤和 8-羟基鸟嘌呤来测定氧化 DNA 损伤。在酸水解小牛胸腺 DNA 中，在 pH 7.2 的磷酸盐缓冲溶液（PBS）中观察到两个明确的峰，约为 312 和 502 mV，分别对应于 8-羟基鸟嘌呤和鸟嘌呤的氧化。与裸 EPPGE 相比，使用 SWNT / EPPGE 时，两种化合物的氧化峰电流均显着增强，峰电位也发生负移。计算出鸟嘌呤和 8-羟基鸟嘌呤的检测限分别为 0.05×10（-9）和 0.01×10（-9）mol L（-1）。对于鸟嘌呤和 8-羟基鸟嘌呤，定量限分别为 0.17×10（-9）和 0.34×10（-10）mol L（-1）。
