State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, People's Republic of China.
Nanotechnology. 2010 Sep 17;21(37):375101. doi: 10.1088/0957-4484/21/37/375101. Epub 2010 Aug 19.
Surface-enhanced Raman scattering (SERS) has been intensely researched for many years as a potential technique for highly sensitive detection. This work, through the reduction of HAuCl(4) with pyrrole in aqueous solutions, investigated a facile one-pot synthesis of flower-like Au nanoparticles with rough surfaces. The formation process of the Au nanoflowers (AuNFs) was carefully studied, and a spontaneous assembly mechanism was proposed based on the time-course experimental results. The key synthesis strategy was to use pyrrole as a weak particle stabilizing and reducing agent to confine crystal growth in the limited ligand protection region. The nanometer-scale surface roughness of AuNFs provided several hot spots on a single particle, which significantly increased SERS enhancement. Good biocompatible stable Raman-active probes were synthesized by coating AuNFs with chitosan. The conservation of the SERS effects in living cells suggested that the chitosan-capped AuNFs could be suitable for highly sensitive detection and have potential for targeting of tumors in vivo.
表面增强拉曼散射（SERS）作为一种高灵敏度检测的潜在技术已经被深入研究多年。这项工作通过在水溶液中用吡咯还原 HAuCl(4)，研究了一种简便的一锅法合成具有粗糙表面的花状金纳米粒子。仔细研究了 Au 纳米花（AuNFs）的形成过程，并基于时程实验结果提出了一种自发组装机制。关键的合成策略是使用吡咯作为弱颗粒稳定剂和还原剂，将晶体生长限制在有限的配体保护区域内。AuNFs 的纳米级表面粗糙度在单个颗粒上提供了多个热点，从而显著提高了 SERS 增强效果。通过用壳聚糖包覆 AuNFs，合成了具有良好生物相容性和稳定的拉曼活性探针。活细胞中 SERS 效应的保持表明，壳聚糖包覆的 AuNFs 可用于高灵敏度检测，并有可能在体内靶向肿瘤。
