用于相干反斯托克斯拉曼散射（CARS）显微镜中非共振背景抑制的三频对称减法方案。

Triple-frequency symmetric subtraction scheme for nonresonant background suppression in coherent anti-Stokes Raman scattering (CARS) microscopy.

作者信息

Fang Chao, Lu Fake, Zheng Wei, Huang Zhiwei

机构信息

Optical Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, National University of Singapore, 117576 Singapore.

出版信息

Opt Express. 2010 Jul 19;18(15):15714-24. doi: 10.1364/OE.18.015714.

DOI:10.1364/OE.18.015714

PMID:20720954

Abstract

We report a unique triple-frequency symmetric subtraction scheme to effectively remove the nonresonant background in coherent anti-Stokes Raman scattering (CARS) microscopy. Theoretical and experimental studies show that this unique scheme has an optimal performance for high contrast vibrational imaging, particularly useful when the resonant signal was larger than or comparable to the nonresonant background.

摘要

我们报告了一种独特的三频对称减法方案，用于在相干反斯托克斯拉曼散射（CARS）显微镜中有效去除非共振背景。理论和实验研究表明，这种独特的方案在高对比度振动成像方面具有最佳性能，当共振信号大于或与非共振背景相当时尤其有用。