School of Biomedical and Health Sciences, Victoria Univ., Melbourne, VIC, Australia.
J Food Sci. 2010 Aug 1;75(6):M373-6. doi: 10.1111/j.1750-3841.2010.01663.x.
The presence of phytate in calcium-fortified soymilk may interfere with mineral absorption. Certain lactic acid bacteria (LAB) produce the enzyme phytase that degrades phytates and therefore may potentially improve mineral bioavailability and absorption. This study investigates the phytase activity and phytate degradation potential of 7 strains of LAB including: Lactobacillus acidophilus ATCC4962, ATCC33200, ATCC4356, ATCC4161, L. casei ASCC290, L. plantarum ASCC276, and L. fermentum VRI-003. Activity of these bacteria was examined both in screening media and in calcium-fortified soymilk supplemented with potassium phytate. Most strains produced phytase under both conditions with L. acidophilus ATCC4161 showing the highest activity. Phytase activity in fortified soymilk fermented with L. acidophilus ATCC4962 and L. acidophilus ATCC4161 increased by 85% and 91%, respectively, between 12 h and 24 h of fermentation. All strains expressed peak phytase activity at approximately pH 5. However, no phytate degradation could be observed.
大豆乳钙强化食品中的植酸可能会干扰矿物质的吸收。某些乳酸菌（LAB）会产生植酸酶，这种酶可以降解植酸，从而可能提高矿物质的生物利用度和吸收率。本研究调查了 7 株 LAB 菌株的植酸酶活性和植酸降解潜力，包括嗜酸乳杆菌 ATCC4962、ATCC33200、ATCC4356、ATCC4161、干酪乳杆菌 ASCC290、植物乳杆菌 ASCC276 和发酵乳杆菌 VRI-003。在筛选培养基和添加钾植酸的钙强化大豆乳中检测了这些细菌的活性。大多数菌株在这两种条件下都产生了植酸酶，其中嗜酸乳杆菌 ATCC4161 的活性最高。在添加钙的大豆乳中发酵 12 小时至 24 小时期间，用嗜酸乳杆菌 ATCC4962 和嗜酸乳杆菌 ATCC4161 发酵的大豆乳中的植酸酶活性分别增加了 85%和 91%。所有菌株在大约 pH5 时表达出最大的植酸酶活性。然而，没有观察到植酸的降解。
