Departamento de Tecnología de Alimentos, INIA, Carretera de la Coruña Km 7, Madrid, 28040 Spain.
J Dairy Sci. 2010 Sep;93(9):3965-9. doi: 10.3168/jds.2010-3152.
The antimicrobial effect of bovine lactoferrin (LF) and its amidated and pepsin-digested derivatives, at concentrations varying from 0.25 to 20 mg/mL, against 3 Salmonella Enteritidis strains and 3 Pseudomonas fluorescens strains was investigated. Lactoferrin showed its maximum antimicrobial effect at 10 mg/mL against the 3 Salmonella strains, with reductions ranging from 1.3 to 2.0 log units, and the 3 Pseudomonas strains, with reductions ranging from 1.8 to 5.4 log units. In the case of amidated LF, the maximum effect on the 3 Salmonella strains was recorded at 0.25 mg/mL, with reductions in the range of 0.8 to 1.2 log units, whereas it was recorded at 1 mg/mL for the 3 Pseudomonas strains, with reductions in the range of 4.4 to 6.0 log units. Pepsin-digested LF showed its maximum antimicrobial effect at 1 mg/mL against the 3 Salmonella strains, with reductions ranging from 2.6 to 3.4 log units, and at 20 mg/mL against the 3 Pseudomonas strains, with reductions ranging from 4.5 to 5.4 log units. It is worth noting the pronounced effect (reductions exceeding 2.5 log units) of a low (1 mg/mL) concentration of pepsin-digested LF, which is naturally formed in the gastrointestinal tract, on Salmonella and Pseudomonas strains. A highly significant inverse correlation was found between capsule polysaccharide levels of bacterial strains and their lethality in the presence of different concentrations of amidated lactoferrin.
研究了牛乳铁蛋白（LF）及其酰胺化和胃蛋白酶消化衍生物在 0.25 至 20 mg/mL 浓度下对 3 株肠炎沙门氏菌和 3 株荧光假单胞菌的抗菌作用。乳铁蛋白在 10 mg/mL 时对 3 株肠炎沙门氏菌表现出最大的抗菌效果，减少幅度为 1.3 至 2.0 对数单位，对 3 株荧光假单胞菌的减少幅度为 1.8 至 5.4 对数单位。对于酰胺化 LF，对 3 株肠炎沙门氏菌的最大作用在 0.25 mg/mL 时记录，减少幅度在 0.8 至 1.2 对数单位范围内，而对 3 株荧光假单胞菌的最大作用在 1 mg/mL 时记录，减少幅度在 4.4 至 6.0 对数单位范围内。胃蛋白酶消化 LF 在 1 mg/mL 时对 3 株肠炎沙门氏菌表现出最大的抗菌效果，减少幅度在 2.6 至 3.4 对数单位范围内，在 20 mg/mL 时对 3 株荧光假单胞菌表现出最大的抗菌效果，减少幅度在 4.5 至 5.4 对数单位范围内。值得注意的是，在胃肠道中自然形成的低浓度（1 mg/mL）胃蛋白酶消化 LF 对肠炎沙门氏菌和荧光假单胞菌菌株具有明显的作用（减少超过 2.5 对数单位）。还发现细菌菌株荚膜多糖水平与其在不同浓度酰胺化乳铁蛋白存在下的致死率之间存在高度显著的负相关。
