原生和受干扰草原溪流中原位反硝化活性与nir 基因丰度的相关性。
Correlations between in situ denitrification activity and nir-gene abundances in pristine and impacted prairie streams.
机构信息
Department of Civil, Environmental and Architectural Engineering, University of Kansas, Lawrence, KS 66045, USA.
出版信息
Environ Pollut. 2010 Oct;158(10):3225-9. doi: 10.1016/j.envpol.2010.07.010. Epub 2010 Aug 17.
Denitrification is a process that reduces nitrogen levels in headwaters and other streams. We compared nirS and nirK abundances with the absolute rate of denitrification, the longitudinal coefficient of denitrification (i.e., Kden, which represents optimal denitrification rates at given environmental conditions), and water quality in seven prairie streams to determine if nir-gene abundances explain denitrification activity. Previous work showed that absolute rates of denitrification correlate with nitrate levels; however, no correlation has been found for denitrification efficiency, which we hypothesise might be related to gene abundances. Water-column nitrate and soluble-reactive phosphorus levels significantly correlated with absolute rates of denitrification, but nir-gene abundances did not. However, nirS and nirK abundances significantly correlated with Kden, as well as phosphorus, although no correlation was found between Kden and nitrate. These data confirm that absolute denitrification rates are controlled by nitrate load, but intrinsic denitrification efficiency is linked to nirS and nirK gene abundances.
反硝化作用是一种降低水源和其他溪流中氮含量的过程。我们比较了nirS 和 nirK 的丰度与绝对反硝化速率、反硝化纵向系数（即 Kden，代表给定环境条件下的最佳反硝化速率）以及 7 条草原溪流的水质，以确定nir 基因丰度是否能解释反硝化活性。先前的工作表明，绝对反硝化速率与硝酸盐水平相关；然而，对于反硝化效率没有发现相关性，我们假设这可能与基因丰度有关。水柱硝酸盐和可溶性反应磷水平与绝对反硝化速率显著相关，但 nir 基因丰度则没有。然而，nirS 和 nirK 的丰度与 Kden 以及磷显著相关，尽管 Kden 与硝酸盐之间没有相关性。这些数据证实，绝对反硝化速率受硝酸盐负荷控制，但内在反硝化效率与 nirS 和 nirK 基因丰度有关。
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