增溶环境对膜蛋白结构的影响。
Influence of solubilizing environments on membrane protein structures.
机构信息
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA.
出版信息
Trends Biochem Sci. 2011 Feb;36(2):117-25. doi: 10.1016/j.tibs.2010.07.005. Epub 2010 Aug 18.
Abstract
Membrane protein structures are stabilized by weak interactions and are influenced by additional interactions with the solubilizing environment. Structures of influenza virus A M2 protein, a proven drug target, have been determined in three different environments, thus providing a unique opportunity to assess environmental influences. Structures determined in detergents and detergent micelles can have notable differences from those determined in lipid bilayers. These differences make it imperative to validate membrane protein structures.
摘要
膜蛋白结构由弱相互作用稳定，并受到与溶解环境的额外相互作用的影响。已在三种不同环境中确定了甲型流感病毒 M2 蛋白的结构，这为评估环境影响提供了独特的机会。在去污剂和去污剂胶束中确定的结构与在脂质双层中确定的结构可能有显著差异。这些差异使得验证膜蛋白结构势在必行。
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