Wang Kebo, Wang Chaoxu, Liu Baosheng, Jiang Shan
Department of Nutrition and Food Hygiene, College of Public Health, Harbin Medical University, Harbin 150081, China.
Wei Sheng Yan Jiu. 2010 Jul;39(4):426-9.
To study the effect of copper deficiency on the nutritional status of iron, the expression of hepcidin mRNA and transferrin receptor mRNA in rats.
Forty eight clean male SD rats were randomly divided into four groups according to body weight; and there were 12 rats in each group. The groups are normal iron and copper control group (group I), normal iron and copper deficiency group (group II), normal iron and copper slightly deficient group (group III), both iron and copper slightly deficient group (group IV). Serum, liver and spleen of rats were collected by the end of 8th week. Serum copper, serum iron, hemoglobin, serum transferrin receptor, serum ferritin, liver iron and liver copper, spleen iron and spleen copper were determined. The expression of liver transferring receptor mRNA and hepcidin mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR) method.
Compared with the controls, the contents of serum iron and serum ferritin decreased (P < 0.05), but the level of serum transferrin receptor and the content of iron in liver and spleen increased significantly under copper deficiency. The expression of transferrin receptor mRNA in liver increased but the expression of hepcidin mRNA in liver decreased significantly under copper deficiency (P < 0.05).
The nutritional status of iron may be affected by copper deficiency through influencing the absorption, storage and transportation of iron. Under the condition of copper deficiency, the expression of hepcidin mRNA in liver was lowered and the expression of transferrin receptor mRNA was enhanced through the way of iron response element-iron regulatory protein (IRE-IRP) to regulate iron metabolism.
研究铜缺乏对大鼠铁营养状况、肝脏铁调素mRNA及转铁蛋白受体mRNA表达的影响。
48只清洁级雄性SD大鼠按体重随机分为4组，每组12只。分别为正常铁铜对照组（Ⅰ组）、单纯铜缺乏组（Ⅱ组）、轻度铜缺乏组（Ⅲ组）、铁铜轻度缺乏组（Ⅳ组）。实验第8周结束时采集大鼠血清、肝脏和脾脏。检测血清铜、血清铁、血红蛋白、血清转铁蛋白受体、血清铁蛋白、肝脏铁和肝脏铜、脾脏铁和脾脏铜含量。采用逆转录聚合酶链反应（RT-PCR）法检测肝脏转铁蛋白受体mRNA和铁调素mRNA表达。
与对照组相比，铜缺乏时血清铁和血清铁蛋白含量降低（P<0.05），血清转铁蛋白受体水平及肝脏和脾脏铁含量显著升高。铜缺乏时肝脏转铁蛋白受体mRNA表达增加，肝脏铁调素mRNA表达显著降低（P<0.05）。
铜缺乏可能通过影响铁的吸收、储存和转运而影响铁的营养状况。铜缺乏时，肝脏铁调素mRNA表达降低，转铁蛋白受体mRNA表达增强，通过铁反应元件-铁调节蛋白（IRE-IRP）途径调节铁代谢。
