Xu Longyan, Yuan Muyun, Liu Jingyu, Zhao Guiming, Zou Zhifei, Ling Li, Zhang Wang, Jiao Hong
Food Laboratory of Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China.
Wei Sheng Yan Jiu. 2010 Jul;39(4):509-12.
To investigate the molecular type and genetic correlation of Listeria monocytogenes (LMO) isolated from imported and exported foods.
Pulsed-field gel electrophoresis (PFGE) standard method recommended by american PulseNet for typing LMO was applied. The chromosomal DNA of L. monocytogenes was digested by restriction enzyme Asc I and analyzed by PFGE. The PFGE patterns of L. monocytogenes strains isolated from different areas were compared by using BioNumberics software to analyze the similarity between strains.
Twenty-four L. monocytogenes strains were grouped into 20 PFGE subtypes by the similarity of PFGE pattern ranged from 44.45% to 100%. Particularly, it was identified that there were three subtype strain groups, each group sharing with the same PFGE pattern, and the similarity reaching 100%: the first group including LMO16 from Canada, LMO18 from America and LMO13 from Denmark, the second group including LMO7 from Denmark and LMO22 from Shunde, and the third group including LMO21 from Guangzhou and LMO8 from Shanxi.
Generally, much genetic diversity was shown in Listeria monocytogenes isolated from import and export foods, but the correlations among strains are existed to some extent.
调查进出口食品中分离出的单核细胞增生李斯特菌（LMO）的分子类型及遗传相关性。
采用美国PulseNet推荐的脉冲场凝胶电泳（PFGE）标准方法对LMO进行分型。用限制性内切酶Asc I消化单核细胞增生李斯特菌的染色体DNA，并通过PFGE进行分析。利用BioNumberics软件比较不同地区分离出的单核细胞增生李斯特菌菌株的PFGE图谱，分析菌株之间的相似性。
24株单核细胞增生李斯特菌菌株根据PFGE图谱相似性分为20个PFGE亚型，相似性范围为44.45%至100%。特别地，鉴定出有三个亚型菌株组，每组具有相同的PFGE图谱，相似性达100%：第一组包括来自加拿大的LMO16、来自美国的LMO18和来自丹麦的LMO13；第二组包括来自丹麦的LMO7和来自顺德的LMO22；第三组包括来自广州的LMO21和来自山西的LMO8。
总体而言，进出口食品中分离出的单核细胞增生李斯特菌表现出较大的遗传多样性，但菌株之间在一定程度上存在相关性。
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