Zhu Ren-fa, Gong Zhao-long, Ye Chang-yun, Cui Zhi-gang, Jin Dong, Ran Lu, Xu Ke, Wang Lian-xiu, Chen Wei-wei
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 May;28(5):464-7.
To analyse the molecular types of Listeria (L.) monocytogenes, and to construct the standard China L. monocytogenes pulsed-field gel electrophoresis (PFGE) subtyping database, using the international standardized L. monocytogenes-PFGE protocol.
118 L. monocytogenes strains isolated from 8 provinces or municipalities of China were subtyped according to L. monocytogenes-PFGE protocol.
118 strains of L. monocytogenes were divided into 39 subtypes. In the 39 subtypes, 37 strains (31.36%) were GX6A160004 pattern, showing it was the predominant Lm subtype in China.
Data from molecular typing suggested that the predominant Lm strains were distributed in different regions of China. PulseNet China-Lm database was constructed which was valuable for the molecular subtyping-based surveillance of L. monocytogenes.
采用国际标准化的单核细胞增生李斯特菌脉冲场凝胶电泳（PFGE）方案，分析单核细胞增生李斯特菌的分子类型，并构建中国单核细胞增生李斯特菌PFGE亚型标准数据库。
按照单核细胞增生李斯特菌PFGE方案，对从中国8个省或直辖市分离出的118株单核细胞增生李斯特菌进行亚型分析。
118株单核细胞增生李斯特菌分为39个亚型。在这39个亚型中，37株（31.36%）为GX6A160004型，表明其为中国单核细胞增生李斯特菌的主要亚型。
分子分型数据表明，主要的单核细胞增生李斯特菌菌株分布于中国不同地区。构建了中国脉冲网-单核细胞增生李斯特菌数据库，该数据库对基于分子亚型的单核细胞增生李斯特菌监测具有重要价值。
