Center for Animal Disease Modeling and Surveillance, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Comp Immunol Microbiol Infect Dis. 2010 Dec;33(6):e119-25. doi: 10.1016/j.cimid.2010.07.001. Epub 2010 Aug 21.
Bison and elk in the greater Yellowstone area are the last-known reservoir of Brucella abortus in the United States. Diagnosis of brucellosis is challenging as there is no perfect reference test. The objectives of this study were to estimate the accuracy of the fluorescence polarization assay (FPA) for the screening of B. abortus antibodies in bison in a natural setting. Serum and tissue samples were collected and analyzed from the known brucellosis-infected bison herd in Yellowstone National Park (YNP). Additionally, serum samples from privately owned bison were serologically tested for brucellosis. While the FPA and five other tests had perfect sensitivity, all tests had substantially lower specificity in the YNP herd. However, a Bayesian analysis showed that as many as 59-74% of the culture-negative animals were most-likely truly infected. A decision-tree analysis showed that the expected cost of FPA testing was comparable to the cost of other serologic tests. The FPA was shown to be highly sensitive but may not be able to differentiate culture-positive and culture-negative animals. There is a need for long-term longitudinal studies to estimate diagnostic accuracy of tests for B. abortus in bison.
大黄石地区的野牛和麋鹿是美国已知的布鲁氏菌流产亚种的最后一个储存库。由于没有完美的参考测试，因此布鲁氏菌病的诊断具有挑战性。本研究的目的是评估荧光偏振测定法（FPA）在自然环境中筛查野牛布鲁氏菌抗体的准确性。从黄石国家公园（YNP）已知的布鲁氏菌病感染野牛群中采集和分析血清和组织样本。此外，对私人拥有的野牛的血清样本进行布鲁氏菌病血清学检测。虽然 FPA 和其他五种测试具有完美的敏感性，但在 YNP 牛群中，所有测试的特异性都大大降低。然而，贝叶斯分析表明，多达 59-74%的培养阴性动物极有可能真正感染。决策树分析表明，FPA 测试的预期成本与其他血清学测试的成本相当。FPA 被证明具有高度敏感性，但可能无法区分培养阳性和培养阴性的动物。需要进行长期纵向研究来估计野牛布鲁氏菌检测的诊断准确性。
