对来自美国蒙大拿州黄石国家公园的美洲野牛（Bison bison）公牛精液进行布鲁氏菌流产亚种排菌情况评估。

Evaluation of bison (Bison bison) semen from Yellowstone National Park, Montana, USA, bulls for Brucella abortus shedding.

作者信息

Frey Rebecca K, Clarke P Ryan, McCollum Matt P, Nol Pauline, Johnson Kammy R, Thompson Brent D, Ramsey Jennifer M, Anderson Neil J, Rhyan Jack C

机构信息

United States Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services (VS) Western Region, 2150 Centre Ave., Building B, Fort Collins, Colorado 80526, USA.

出版信息

J Wildl Dis. 2013 Jul;49(3):714-7. doi: 10.7589/2012-04-118.

DOI:10.7589/2012-04-118

PMID:23778628

Abstract

To determine if bison (Bison bison) bulls from Yellowstone National Park (YNP), Montana, USA, shed an infective dose of Brucella abortus in semen, 50 YNP bulls were captured on public lands in Montana during the winter and early spring (April-May) of 2010 and 2011. The bulls were immobilized, and blood and semen samples were collected for serology and Brucella culture. Thirty-five bulls (70%) were antibody-positive, and B. abortus was cultured from semen in three (9%) of the 35 antibody-positive or suspect bulls, though not at concentrations considered an infective dose. Eight bulls (six antibody-positive, two negative) had palpable lesions of the testes, epididymides, or seminal vesicles consistent with B. abortus infection. Breeding soundness exams and semen analysis suggested that antibody-positive bulls were more likely to have nonviable ejaculate (8/35; 23%) than bulls without detectable antibody (2/15; 13%).

摘要

为确定来自美国蒙大拿州黄石国家公园（YNP）的美洲野牛公牛精液中是否排出感染剂量的牛布鲁氏菌，2010年和2011年冬春时节（4 - 5月），在蒙大拿州的公共土地上捕获了50头YNP公牛。将公牛麻醉后，采集血液和精液样本进行血清学检测和布鲁氏菌培养。35头公牛（70%）抗体呈阳性，在35头抗体阳性或疑似阳性的公牛中，有3头（9%）精液培养出牛布鲁氏菌，但浓度未达到感染剂量。8头公牛（6头抗体阳性，2头阴性）睾丸、附睾或精囊有明显病变，与牛布鲁氏菌感染相符。繁殖能力检查和精液分析表明，抗体阳性的公牛比未检测到抗体的公牛更易出现无活力射精（8/35；23% vs 2/15；13%）。