He Haitao, Tan Yinghui, Yang Maojin
Department of Oral and Maxillofacial Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, People's Republic of China.
J Oral Maxillofac Surg. 2010 Sep;68(9):2047-52. doi: 10.1016/j.joms.2010.02.016. Epub 2010 Jun 17.
The aims of this experiment were to study the effect and possible mechanism of substance P (SP) in the mandibular osteotomy healing process through inferior alveolar nerve (IAN) amputation.
Thirty-two adult China white rabbits were randomly divided into 2 groups (experimental and control). An osteotomy in the left mandible was created and concomitantly the experimental group underwent IAN amputation. The rabbits were sacrificed 7, 14, 21, and 28 days after operation, and specimens were collected and stained with hematoxylin and eosin and for immunohistochemistry to observe the expression of SP in bone callus and the process of osteotomy healing. Semiquantitative analysis on immunohistochemically stained slices was performed using computer image analysis.
There was a larger amount of fibrous callus formation, relatively immature woven bone callus, and a smaller proportion between matured bone callus and woven bone in the group subjected to IAN amputation than in the controls at each stage, especially in the late stages. Immunoreactivities of SP occurred weakly 7 and 14 days after operation and became stronger gradually in the late stage in the experimental group. Stronger immunoreactivities of SP occurred 7 and 14 days after operation and less on day 21 after trauma and became strongest on day 28 after trauma in the control group. The strongest immunoreactivities at each stage occurred on day 28 after trauma in both groups.
SP secreted by IAN may be very important to initiate and modulate the process of repair and remodeling of bone.
本实验旨在通过切断下牙槽神经（IAN）研究P物质（SP）在下颌骨截骨愈合过程中的作用及可能机制。
32只成年中国白兔随机分为2组（实验组和对照组）。在左侧下颌骨制造截骨，同时实验组切断IAN。术后7、14、21和28天处死兔子，收集标本，进行苏木精-伊红染色和免疫组织化学染色，以观察骨痂中SP的表达及截骨愈合过程。使用计算机图像分析对免疫组织化学染色切片进行半定量分析。
在每个阶段，尤其是后期，切断IAN组的纤维性骨痂形成量更多，编织骨痂相对不成熟，成熟骨痂与编织骨的比例更小。实验组术后7天和14天SP免疫反应较弱，后期逐渐增强。对照组术后7天和14天SP免疫反应较强，创伤后21天减弱，创伤后28天最强。两组在每个阶段的最强免疫反应均出现在创伤后28天。
IAN分泌的SP可能对启动和调节骨修复与重塑过程非常重要。
