Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, PR China.
Biosens Bioelectron. 2010 Oct 15;26(2):432-6. doi: 10.1016/j.bios.2010.07.105. Epub 2010 Aug 1.
A Pt nanoparticle-decorated flower-like carbon nanosheet aggregation (FCNA) was prepared via one-step ethylene glycol method. The aggregation was characterized with scanning electron micrographs, X-ray photoelectron spectra, X-ray diffraction and electrochemical impedance spectra. When the aggregation was immobilized on a glassy carbon electrode, the dense dispersion of Pt nanoparticles (Pt NPs) on the carbon nanosheets of FCNA could combine the good conductivity of FCNA with the excellent catalytic activity of Pt NPs for the electroreduction of oxygen at a low overpotential, which led to a method for electrochemical detection of oxygen from 6.3 to 69.3 μM. Using glucose oxidase (GOx) as a model, the resulting GOx/Pt/FCNA nanocomposite-based amperometric biosensor showed a linear response to glucose ranging from 0.5 to 8.0 mM with a detection limit of 0.3 mM at a S/N ratio of 3. The designed biosensor was of excellent performance with high selectivity, acceptable recovery and good repeatability, and could be successfully applied in the detection of glucose in human serum. The FCNA could be expected as a carrier for the preparation of other metal nanoparticle-dispersed aggregations and biosensing applications.
通过一步乙二醇法制备了 Pt 纳米颗粒修饰的花状碳纳米片聚集物（FCNA）。通过扫描电子显微镜、X 射线光电子能谱、X 射线衍射和电化学阻抗谱对聚集物进行了表征。当聚集物固定在玻碳电极上时，FCNA 碳纳米片上 Pt 纳米颗粒（Pt NPs）的密集分散将 FCNA 的良好导电性与 Pt NPs 对低过电势下氧气还原的优异催化活性结合在一起，从而实现了从 6.3 到 69.3 μM 的电化学氧气检测方法。使用葡萄糖氧化酶（GOx）作为模型，基于所得的 GOx/Pt/FCNA 纳米复合材料的安培生物传感器对葡萄糖表现出从 0.5 到 8.0 mM 的线性响应，在 S/N 比为 3 时检测限为 0.3 mM。设计的生物传感器具有出色的性能，具有高选择性、可接受的回收率和良好的重复性，并可成功应用于人血清中葡萄糖的检测。FCNA 有望作为制备其他金属纳米颗粒分散聚集物和生物传感应用的载体。
