Faculty of Science, Department for Chemistry, University of Kragujevac, Kragujevac, Serbia.
Food Chem Toxicol. 2010 Nov;48(11):3125-30. doi: 10.1016/j.fct.2010.08.007. Epub 2010 Aug 20.
The aim of this study was to examine six plants from Serbia for their potential antioxidant activity. Therefore, six antioxidant activity assays were carried out, including: total antioxidant capacity, DPPH free-radical scavenging, the inhibitory activity toward lipid peroxidation, Fe(3+)- reducing power, Fe(2+)- chelating ability and hydroxyl radical scavenging activity. Total phenolic and flavonoid contents were also determined for each alcoholic extract. Cotinus coggygria extract contained the highest amount of total phenols (413mg GAE /g dry extract), while the highest proportion of flavonoids was found in the Echium vulgare methanol extract (105 mg RU/g). Cotinus coggygria and Halacsya sendtneri alcoholic extracts showed the highest total antioxidant capacity (313 and 231 mg AA/g dry extract), as well as DPPH free-radical scavenging (IC(50)=9 and 99 μg/ml), inhibitory activity toward lipid peroxidation (IC(50)=3 and 17 μg/ml) and reducing power. Whereas, the greatest hydroxyl radical scavenging activity, as well as ferrous ion chelating ability showed Echium vulgare, Echium rubrum and Halacsya sendtneri.
本研究旨在考察来自塞尔维亚的六种植物的潜在抗氧化活性。因此,进行了六种抗氧化活性测定,包括:总抗氧化能力、DPPH 自由基清除能力、抑制脂质过氧化能力、Fe(3+)还原能力、Fe(2+)螯合能力和羟基自由基清除能力。还测定了每种醇提物的总酚和总黄酮含量。金缕梅提取物中总酚含量最高(413mg GAE/g 干提取物),而矢车菊甲醇提取物中总黄酮含量最高(105mg RU/g)。金缕梅和Halacsya sendtneri 醇提物的总抗氧化能力最高(313 和 231mg AA/g 干提取物),DPPH 自由基清除能力(IC(50)=9 和 99μg/ml)、抑制脂质过氧化能力(IC(50)=3 和 17μg/ml)和还原能力也最强。而,矢车菊、矢车菊红和 Halacsya sendtneri 的羟基自由基清除能力以及亚铁离子螯合能力最强。