Amrane-Abider Meriem, Imre Mirela, Herman Viorel, Debbou-Iouknane Nedjima, Zemouri-Alioui Salima, Khaled Souad, Bouiche Cilia, Nerín Cristina, Acaroz Ulaș, Ayad Abdelhanine
Centre de Recherche en Technologies Agroalimentaires, Route de Targa Ouzemmour, Campus Universitaire, Bejaia 06000, Algeria.
Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences "King Mihai I" from Timisoara, 300645 Timisoara, Romania.
Biomedicines. 2023 Aug 2;11(8):2173. doi: 10.3390/biomedicines11082173.
The objective of the present study is to identify the biochemical compounds extracted from OFI flowers using ultra-high-performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry and to evaluate their in vitro antioxidant activities and anticoccidial effects on the destruction of oocysts isolated from naturally infected chickens. A domestic microwave was used with a refrigerant to condense the vapors generated during the extraction. The flavonoid and phenolic compound contents of the OFI flowers were determined according to standard methods. DPPH radical and HO scavenging capacities were used to assess the antioxidant activity. Regarding the anticoccidial activity, the spp. oocysts used were isolated from the fresh feces of infected broilers and were determined in triplicate by incubation at an ambient temperature for 24 h. The results highlighted the considerable influence of the optimized acetone concentration, ratio, irradiation time, and microwave power parameters on the phenolic content and antioxidant activities. Our results revealed significant matches between the predicted and experimental values of the models. Molecular analysis revealed the presence of several biophenol classes such as quercetin, isorhamnetin 3-O-rutinoside, and quercetin-3-O-rutinoside. OFI flower extracts inhibited sporulation and damaged the morphology of oocysts compared with normal sporulated oocysts containing sporocysts. In conclusion, the optimized conditions were validated and found to fit very well with the experimental values. These findings suggest that the flowers of OFI should be considered sources of antioxidants. The results of the present study revealed that OFI flower extracts have anticoccidial activities against -spp.-induced infection in broiler chickens.
本研究的目的是利用超高效液相色谱-电喷雾电离四极杆飞行时间质谱法鉴定从OF I花中提取的生化化合物，并评估其体外抗氧化活性以及对从自然感染鸡中分离出的卵囊破坏的抗球虫作用。使用家用微波炉并配备制冷剂来冷凝提取过程中产生的蒸汽。根据标准方法测定OF I花中的黄酮类化合物和酚类化合物含量。采用DPPH自由基和羟基清除能力来评估抗氧化活性。关于抗球虫活性，所用的球虫卵囊是从感染肉鸡的新鲜粪便中分离出来的，并在环境温度下孵育24小时进行三次重复测定。结果突出了优化的丙酮浓度、比例、辐照时间和微波功率参数对酚类含量和抗氧化活性的显著影响。我们的结果显示模型的预测值与实验值之间存在显著匹配。分子分析揭示了几种生物酚类的存在，如槲皮素、异鼠李素3-O-芸香糖苷和槲皮素-3-O-芸香糖苷。与含有子孢子囊的正常孢子化卵囊相比，OF I花提取物抑制了孢子形成并破坏了卵囊的形态。总之，优化条件得到了验证，发现与实验值非常吻合。这些发现表明OF I花应被视为抗氧化剂的来源。本研究结果表明，OF I花提取物对肉鸡中由球虫属引起的感染具有抗球虫活性。
