Urios Laurent, Intertaglia Laurent, Lesongeur Françoise, Lebaron Philippe
CNRS, UMR 7621, Laboratoire d'Océanographie Microbienne, Avenue Fontaulé, F-66650 Banyuls-sur-Mer, France.
Université Pierre et Marie Curie-Paris 6, Laboratoire ARAGO, Avenue Fontaulé, F-66650 Banyuls-sur-Mer, France.
Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1677-1681. doi: 10.1099/ijs.0.023952-0. Epub 2010 Aug 20.
A novel aerobic, gram-negative bacterial strain, designated 17X/A02/237(T), was isolated from waters of the coastal north-western Mediterranean Sea. Cells were motile straight rods and formed dark-grey colonies on marine agar medium. Strain 17X/A02/237(T) contained ubiquinone Q-8 and its major fatty acids were C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH, C(18 : 1)ω7c, C(16 : 0), C(18 : 0) and C(10 : 0) 3-OH. The G+C content of the genomic DNA was 47.5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the class Gammaproteobacteria. Based on 16S rRNA gene sequence data, as well as physiological and biochemical characteristics, this isolate represents a novel species of a new genus, for which the name of Eionea nigra gen. nov., sp. nov. is proposed. The type strain is 17X/A02/237(T) ( = DSM 19752(T) = CIP 109759(T) = MOLA 288(T)).
从地中海西北部沿海海域分离出一株新型需氧革兰氏阴性细菌菌株,命名为17X/A02/237(T)。细胞为运动性直杆状,在海洋琼脂培养基上形成深灰色菌落。菌株17X/A02/237(T)含有泛醌Q-8,其主要脂肪酸为C(16 : 1)ω7c和/或异-C(15 : 0) 2-OH、C(18 : 1)ω7c、C(16 : 0)、C(18 : 0)和C(10 : 0) 3-OH。基因组DNA的G+C含量为47.5 mol%。对16S rRNA基因序列进行系统发育分析,该菌株属于γ-变形菌纲。基于16S rRNA基因序列数据以及生理生化特征,该分离物代表一个新属的新物种,为此提出新属名为Eionea nigra gen. nov.,新种名为Eionea nigra sp. nov.。模式菌株为17X/A02/237(T)(=DSM 19752(T)=CIP 109759(T)=MOLA 288(T))。