Sofikitis N, Takahaski C, Nakamura I, Kadowaki H, Okazaki T, Shimamoto T, Miyagawa I
Department of Urology, Tottori University Medical School, Yonago, Japan.
Acta Eur Fertil. 1990 May-Jun;21(3):155-8.
To investigate the contribution of the rat copulatory plug (RCP) to fertilization we evaluated the following: 1) its role as a reservoir for spermatozoa, 2) its role in the prevention of the outflow of spermatozoa from the vagina, and 3) its importance in the process of the sperm into the uterus. Artificial inseminations with either fresh RCP or epididymal spermatozoal solutions or the postcoital removal of the RCP was performed in normal female rats in estrus and in pseudopregnant rats (following relaxation of the cervix by faradic stimulation). The pregnancy rates in the different groups indicated that only the third mechanism was important.
为了研究大鼠交配栓(RCP)对受精的作用,我们评估了以下几点:1)其作为精子储存库的作用;2)其在防止精子从阴道流出方面的作用;3)其在精子进入子宫过程中的重要性。在处于发情期的正常雌性大鼠和假孕大鼠(通过感应电刺激使子宫颈松弛后)中,分别进行了用新鲜RCP或附睾精子溶液人工授精,或交配后去除RCP的操作。不同组别的妊娠率表明,只有第三种机制是重要的。