Process Analytical Chemistry, Global Pharmaceutical Research & Development, Abbott Laboratories, North Chicago, IL 60064, USA. USA.
J Sep Sci. 2010 Oct;33(19):3060-7. doi: 10.1002/jssc.201000371.
Supercritical fluid chromatography was utilized in combination with the Abraham model of linear solvation energy relationship to characterize 11 different HPLC stationary phases. System constants were determined at one supercritical fluid chromatography condition for each stationary phase. The results indicate that several types of silica columns, including type B silica, type C silica, and fused core silica, are very similar in their retention behavior. Several aromatic stationary phases were characterized and it was found that, in contrast to the other phases studied, all of the aromatic stationary phases had positive contributions from the dispersion/cavity (v) term of the linear solvation energy relationship. Several aliphatic phases were characterized and there were several linear solvation energy relationship constants that differentiated the phases from each other, mainly the polar terms (dipolarity and hydrogen bonding). One stationary phase, a fused core pentafluorophenyl (PFP) phase, had very poor regression quality. The column volume of this phase was lower than the others in the study, which may have had some impact on the results of the regression.
超临界流体色谱与 Abraham 线性溶剂化能量关系模型相结合，用于表征 11 种不同的 HPLC 固定相。为每个固定相确定了一个超临界流体色谱条件下的系统常数。结果表明，几种类型的硅胶柱，包括 B 型硅胶、C 型硅胶和熔融核硅胶，在保留行为上非常相似。对几种芳香族固定相进行了表征，结果发现，与研究中的其他相相比，所有的芳香族固定相的线性溶剂化能量关系色散/空穴（v）项都有正贡献。对几种脂肪族相进行了表征，有几个线性溶剂化能量关系常数可以将这些相彼此区分开来，主要是极性项（偶极矩和氢键）。一种固定相，即熔融核五氟苯基（PFP）相，回归质量很差。该相的柱体积低于研究中的其他相，这可能对回归结果有一定影响。
