Microbiology Laboratory, Bioengineering and Environmental Center, Indian Institute of Chemical Technology, Hyderabad, India.
Pharm Biol. 2010 Sep;48(9):1007-11. doi: 10.3109/13880200903433760.
The roots of Plumbago zeylanica Linn. (Plumbaginaceae) are reputed to have a wide spectrum of therapeutic properties in the Ayurvedic system of medicine. They are useful in curing many ailments such as skin diseases, diarrhea, plague and leprosy.
The study was aimed at isolating, separating and evaluating the antimicrobial properties of compounds such as neoisoshinanolone and 1-epineo-isoshinanolone from the roots of P. zeylanica.
The crude petroleum ether extract of roots of P. zeylanica was subjected to repeated chromatographic techniques to separate compounds 2 and 3 along with plumbagin. Structure elucidation was carried out using nuclear magnetic resonance (NMR), infra red (IR) and mass spectroscopy. The serial dilution method was used to test antimicrobial activities and their minimum inhibitory concentration (MIC) expressed in microg/mL.
1-Epineo-isoshinanolone is more active with a MIC of 12.5-25 microg/mL whereas neoisoshinanolone has recorded a MIC of 50-100 microg/mL. The activities are compared with plumbagin (0.78-3.13 microg/mL) and standards streptomycin for bacteria and nystatin for fungi.
Earlier researchers have established the presence of plumbagin in the roots of P. zeylanica and its antimicrobial activities. The structure elucidation of two more biologically active biogenetic precursors along with their activities in the root extracts has been established for the first time in the present study.
The root extract of P. zeylanica possesses good antimicrobial activity, which suggests its therapeutic use in the Ayurvedic system of medicine to cure skin diseases.
印度菥蓂（蓝雪科）的根在印度阿育吠陀医学体系中被认为具有广泛的治疗特性。它们可用于治疗多种疾病，如皮肤病、腹泻、瘟疫和麻风病。
本研究旨在从印度菥蓂的根部分离、分离和评估化合物的抗菌特性，如 neoishosinanolone 和 1-epineo-isoshinanolone。
印度菥蓂根的粗石油醚提取物经过反复的色谱技术处理，分离出化合物 2 和 3 以及白花丹醌。通过核磁共振（NMR）、红外（IR）和质谱对结构进行了阐明。采用连续稀释法测试抗菌活性及其以微克/毫升表示的最小抑菌浓度（MIC）。
1-epineo-isoshinanolone 的活性更强，MIC 为 12.5-25 微克/毫升，而 neoishosinanolone 的 MIC 为 50-100 微克/毫升。与白花丹醌（0.78-3.13 微克/毫升）和细菌标准品链霉素以及真菌标准品制霉菌素进行比较。
早期研究人员已经确定了印度菥蓂根中存在白花丹醌及其抗菌活性。本研究首次对两种更具生物活性的生物合成前体的结构进行了阐明，并确定了它们在根提取物中的活性。
印度菥蓂根提取物具有良好的抗菌活性，这表明其在印度阿育吠陀医学体系中用于治疗皮肤病的治疗用途。
