细胞色素氧化酶活性极谱测量中的异常情况。

Anomalies in the polarographic measurement of cytochrome oxidase activity.

作者信息

Wiest S C, Steponkus P L

出版信息

Biochim Biophys Acta. 1978 May 10;502(2):248-54. doi: 10.1016/0005-2728(78)90046-4.

PMID:207317

Abstract

Reaction kinetics of the reduction of O2 by cytochrome oxidase follow essentially the same rate equation as that proposed for the oxidation of cytochrome c. However, the apparent second order rate constant varies with the oxidase concentration. The redox level of cytochrome c at the steady state was found to be essentially temperature-independent. Currently recognized pathways (or mechanisms) of electron transport from cytochrome c to O2 do not predict, and cannot account for the occurrence of these phenomena.

摘要

细胞色素氧化酶还原O₂的反应动力学基本上遵循与细胞色素c氧化反应所提出的相同速率方程。然而，表观二级速率常数随氧化酶浓度而变化。发现细胞色素c在稳态下的氧化还原水平基本与温度无关。目前公认的从细胞色素c到O₂的电子传递途径（或机制）无法预测，也不能解释这些现象的发生。