Speck S H, Dye D, Margoliash E
Proc Natl Acad Sci U S A. 1984 Jan;81(2):347-51. doi: 10.1073/pnas.81.2.347.
A single catalytic site model is proposed to account for the multiphasic kinetics of oxidation of ferrocytochrome c by cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1). This model involves nonproductive binding of substrate to sites near the catalytic site on cytochrome c oxidase for cytochrome c, decreasing the binding constant for cytochrome c at the catalytic site. This substrate inhibition results in an increase in the first-order rate constant for the dissociation of the ferricytochrome c-cytochrome c oxidase complex, the rate-limiting step in the steady-state turnover of electrons between cytochrome c and cytochrome c oxidase in the spectrophotometric assay, yielding increases in the initial rate as well as the Michaelis constant--namely, multiple kinetic phases.
提出了一个单一催化位点模型来解释细胞色素c氧化酶氧化亚铁细胞色素c的多相动力学（亚铁细胞色素c：氧氧化还原酶，EC 1.9.3.1）。该模型涉及底物与细胞色素c氧化酶上细胞色素c催化位点附近的位点发生非生产性结合，从而降低细胞色素c在催化位点的结合常数。这种底物抑制导致高铁细胞色素c-细胞色素c氧化酶复合物解离的一级速率常数增加，这是分光光度法测定中细胞色素c和细胞色素c氧化酶之间稳态电子传递的限速步骤，从而导致初始速率以及米氏常数增加——即多个动力学阶段。
