Herno V, Descampiaux B, Imbenotte M, Pommery J, Marzin D, Erb F
Département de Toxicologie, Hydrologie, Hygiène, Faculté des Sciences Pharmaceutiques et Biologiques, BP 83.59006 Lille cédex, France.
Toxicol In Vitro. 1992 Nov;6(6):519-22. doi: 10.1016/0887-2333(92)90063-w.
In a preliminary investigation, the evolution with time of the levels of ATP and glutathione species [reduced glutathione (GSH) and glutathione disulphide (GSSG)], glutathione reductase activity and RNA synthesis rate were studied in two human hepatoma cell lines, Hep 3B and Hep G2. Both cell lines exhibited higher cellular activities, during the first 48 hr. In a second investigation, the cells were exposed to lindane. Different cytotoxicological parameters such as viability, lactate dehydrogenase release, ATP, GSH and GSSG contents, RNA synthesis rate and glutathione reductase activity, were measured. Results showed that Hep 3B cells were more susceptible to lindane than Hep G2 cells, which perhaps have superior defence capacity and metabolism.
在一项初步研究中，对两种人肝癌细胞系Hep 3B和Hep G2中ATP和谷胱甘肽种类[还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）]的水平、谷胱甘肽还原酶活性以及RNA合成速率随时间的变化进行了研究。在最初的48小时内，两种细胞系均表现出较高的细胞活性。在第二项研究中，将细胞暴露于林丹中。测量了不同的细胞毒理学参数，如活力、乳酸脱氢酶释放量、ATP、GSH和GSSG含量、RNA合成速率以及谷胱甘肽还原酶活性。结果表明，Hep 3B细胞比Hep G2细胞对林丹更敏感，后者可能具有更强的防御能力和代谢能力。
