Center for Molecular Virology, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
J Clin Virol. 2010 Nov;49(3):186-91. doi: 10.1016/j.jcv.2010.07.018. Epub 2010 Aug 21.
H1N1 is the main subtype influenza A virus circulating in human and swine population, and has long been a threat to economy and public health.
To explore the genetic correlation between current circulating H1N1 swine and human influenza viruses.
Three new H1N1 swine influenza viruses (SIVs) were isolated and genomes sequencing were conducted followed by phylogenetic and molecular analysis of all swine and human H1N1 influenza viruses isolated in China in the past five years.
Homology and phylogenetic analysis revealed that the three isolates possessed different characteristics: the genome of A/Swine/Shandong/1112/2008 was closely related to that of classical H1N1 SIV, while A/Swine/Shandong/1123/2008 was a reassortant with NS gene from the human-like H3N2 influenza virus and other genes from the classical H1N1 SIV, and A/Swine/Fujian/0325/2008 fell into a lineage of seasonal human H1N1 influenza viruses. Genetically, 2009 H1N1 influenza A viruses (2009 H1N1) in China were contiguous to the SIV lineages rather than the seasonal H1N1 human influenza virus's lineage. Furthermore, molecular analysis among human and swine influenza viruses provided more detail information for understanding their genetic correlation.
These results suggested that in China in the past five years, the classical, avian-like and human-like H1N1 SIV existed in swine herds and the reassortment between H1N1 swine and H3N2 human influenza viruses was identified. In addition, the present data showed no evidence to support a strong correlation between the 2009 H1N1 and the swine influenza virus circulating in China.
H1N1 是在人类和猪群中流行的主要甲型流感病毒亚型，长期以来一直是经济和公共卫生的威胁。
探索当前流行的 H1N1 猪流感病毒与人类流感病毒之间的遗传相关性。
分离了 3 株新的 H1N1 猪流感病毒（SIV），并对其基因组进行测序，随后对过去 5 年在中国分离的所有猪和人类 H1N1 流感病毒进行了系统发育和分子分析。
同源性和系统发育分析表明，这 3 个分离株具有不同的特征：A/Swine/Shandong/1112/2008 株的基因组与经典 H1N1 SIV 密切相关，而 A/Swine/Shandong/1123/2008 株是一种重配株，其 NS 基因来自人源 H3N2 流感病毒，其他基因来自经典 H1N1 SIV，A/Swine/Fujian/0325/2008 株则属于季节性人源 H1N1 流感病毒谱系。遗传上，中国的 2009 年 H1N1 流感病毒（2009 H1N1）与 SIV 谱系而非季节性 H1N1 人源流感病毒谱系相连。此外，对人源和猪源流感病毒的分子分析为了解它们的遗传相关性提供了更详细的信息。
这些结果表明，在过去的 5 年中，中国的猪群中存在经典、禽源和人源 H1N1 SIV，并且鉴定了 H1N1 猪流感病毒与 H3N2 人源流感病毒之间的重配。此外，目前的数据没有证据支持 2009 年 H1N1 与中国流行的猪流感病毒之间存在很强的相关性。
