Area de Química Analítica, Universitat Jaume I, Campus Riu Sec, 12071 Castelló, Spain.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Sep 15;878(26):2397-402. doi: 10.1016/j.jchromb.2010.07.010. Epub 2010 Jul 27.
Nicotine can be determined in pharmaceuticals and biological fluids by micellar liquid chromatography (MLC) using a C18 column, a mobile phase containing sodium dodecyl sulphate 0.15M-6% (v/v) pentanol-NaH(2)PO(4) 0.01 M (pH 6)-KCl 0.001 M, with electrochemical detection at 0.8 V. In the optimization step, the influence of the modifiers propanol, butanol and pentanol, and the voltage has been studied. With the proposed method the analysis time is below than 8 min, linearity better than 0.999, limits of detection and quantification (ng/ml) was 4 and 12 respectively, repeatability and intermediate precision below 1.8%, and all these parameters are adequate for the quantification of nicotine in chewing gum, dermal patches, tobacco and serum samples either by a pharmacologist, pathologist or toxicologist.
尼古丁可通过胶束液相色谱法（MLC）用 C18 柱、包含 0.15M-6%（v/v）戊醇-十二烷基硫酸钠、0.01M 磷酸氢二钠- 戊醇-NaH(2)PO(4)（pH6）-0.001M 氯化钾的流动相，在 0.8V 下进行电化学检测来检测药物和生物液体中的尼古丁。在优化步骤中，研究了改性剂丙醇、丁醇和戊醇以及电压的影响。采用所提出的方法，分析时间小于 8 分钟，线性度优于 0.999，检测限和定量限（ng/ml）分别为 4 和 12，重复性和中间精密度低于 1.8%，所有这些参数都足以用于药剂师、病理学家或毒理学家对咀嚼胶、皮肤贴片、烟草和血清样本中的尼古丁进行定量。
