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3
Immunolabeling for scanning electron microscopy (SEM) and field emission SEM.用于扫描电子显微镜（SEM）和场发射扫描电子显微镜的免疫标记。
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Targeted delivery of progenitor cells for cartilage repair.
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Three-dimensional architecture of ribosomal DNA within barley nucleoli revealed with electron microscopy.
Scanning. 2003 Sep-Oct;25(5):257-63. doi: 10.1002/sca.4950250507.
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7
Mapping gold-labeled IgE receptors on mast cells by scanning electron microscopy: receptor distributions revealed by silver enhancement, backscattered electron imaging, and digital image analysis.通过扫描电子显微镜对肥大细胞上的金标免疫球蛋白E受体进行定位：银增强、背散射电子成像和数字图像分析揭示的受体分布
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一种使用蛋白G偶联聚苯乙烯微球进行大规模免疫扫描电子显微镜的新方法。

A novel method for large-scale immuno-SEM using protein G coupled polystyrene beads.

作者信息

Song In-Hwan, Dennis James E

机构信息

Department of Anatomy, College of Medicine, Yeungnam University, 317-1 Daemyungdong, Daegu 705-717, South Korea.

出版信息

J Electron Microsc (Tokyo). 2010;59(6):527-30. doi: 10.1093/jmicro/dfq064. Epub 2010 Aug 23.

DOI:10.1093/jmicro/dfq064
PMID:20732882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3156677/
Abstract

Polystyrene beads with a mean diameter of 0.76 μm were coupled with protein G and then anti-type II collagen IgG or anti-chondroitin-4-sulphate IgG were tagged to protein G. Antibody-tagged beads were applied to articular cartilage and labelled beads were counted in each sample. Antibody-tagged beads labelled significantly higher than IgG isotype control. We propose immuno-SEM using protein G coupled beads as a valuable method for micrometre range observation for specific protein distribution on surfaces of tissues or organs. This will provide information about structure as well as antigenicity on the surface at the same time.

摘要

将平均直径为0.76μm的聚苯乙烯珠与蛋白G偶联，然后将抗II型胶原IgG或抗硫酸软骨素-4-硫酸酯IgG标记到蛋白G上。将抗体标记的珠子应用于关节软骨，并对每个样本中的标记珠子进行计数。抗体标记的珠子标记显著高于IgG同型对照。我们提出使用蛋白G偶联珠的免疫扫描电子显微镜作为一种有价值的方法，用于在微米范围内观察组织或器官表面的特定蛋白质分布。这将同时提供有关表面结构和抗原性的信息。