Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
Philos Trans A Math Phys Eng Sci. 2010 Sep 28;368(1927):4419-37. doi: 10.1098/rsta.2010.0133.
Quadrupole magnetic field-flow fractionation is a relatively new technique for the separation and characterization of magnetic nanoparticles. Magnetic nanoparticles are often of composite nature having a magnetic component, which may be a very finely divided material, and a polymeric or other material coating that incorporates this magnetic material and stabilizes the particles in suspension. There may be other components such as antibodies on the surface for specific binding to biological cells, or chemotherapeutic drugs for magnetic drug delivery. Magnetic field-flow fractionation (MgFFF) has the potential for determining the distribution of the magnetic material among the particles in a given sample. MgFFF differs from most other forms of field-flow fractionation in that the magnetic field that brings about particle separation induces magnetic dipole moments in the nanoparticles, and these potentially can interact with one another and perturb the separation. This aspect is examined in the present work. Samples of magnetic nanoparticles were analysed under different experimental conditions to determine the sensitivity of the method to variation of conditions. The results are shown to be consistent and insensitive to conditions, although magnetite content appeared to be somewhat higher than expected.
四极磁场流分离是一种用于分离和表征磁性纳米粒子的相对较新的技术。磁性纳米粒子通常具有复合性质，具有磁性成分，该磁性成分可能是非常细的材料，以及涂覆有这种磁性材料并使颗粒在悬浮液中稳定的聚合物或其他材料。表面上可能有其他成分，如针对生物细胞的特异性结合的抗体，或用于磁性药物递送的化疗药物。磁场流分离（MgFFF）有可能确定给定样品中磁性材料在颗粒中的分布。MgFFF 与大多数其他形式的场流分离不同，因为引起颗粒分离的磁场会在纳米颗粒中诱导磁偶极矩，并且这些偶极矩可能会相互作用并干扰分离。本工作对此方面进行了研究。在不同的实验条件下分析了磁性纳米粒子的样品，以确定该方法对条件变化的敏感性。结果表明，该方法的结果是一致的，并且对条件不敏感，尽管磁铁矿含量似乎比预期的略高。
