Chirpich T P
Biochim Biophys Acta. 1978 May 23;518(3):535-8. doi: 10.1016/0005-2787(78)90172-7.
The polymerization of dATP, dCTP, and dGTP onto the defined length initiator, d(pA)10, has been carried out in four buffers. The relative effectiveness of the buffers for the polymerization of each deoxynucleoside triphosphate decreased in the order: cacodylate, 2(N-morpholino)ethane sulfonic acid, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, and Tris. With the poorer buffers, activity could be increased by the addition of KCl: this effect is not primarily due to an increase in ionic strength. With dGTP as the substrate, but not with dATP or dCTP, activity increased when the concentration of the more active buffers was raised beyond 0.2 M.
已在四种缓冲液中进行了dATP、dCTP和dGTP在确定长度的引发剂d(pA)10上的聚合反应。这些缓冲液对每种脱氧核苷三磷酸聚合反应的相对有效性按以下顺序降低：二甲胂酸盐、2-（N-吗啉代）乙磺酸、N-2-羟乙基哌嗪-N'-2-乙磺酸和Tris。对于较差的缓冲液，添加KCl可提高活性：这种效应主要不是由于离子强度的增加。以dGTP作为底物时，活性会增加，但以dATP或dCTP作为底物时则不会，当活性较高的缓冲液浓度提高到超过0.2 M时，活性会增加。
