Braggio Morucchio G, Cornara L, Dellachà E
Istituto di Botanica dell'Università di Genova.
Boll Soc Ital Biol Sper. 1990 Sep;66(9):821-7.
The viability of pollen at room temperature lowers and disappears in few days; on the contrary the cryopreservation lengthens the pollen viability. Pollen of Pinus brutia, P. canariensis, P. halepensis, P. pinaster has been stored at -20 degrees C for one year with the aim of checking the variation of viability. The viability has been tested by fluorescein diacetate; the germination by in vitro culture for 96 hours has been observed and daily percentage of germinated pollen grains and maximum lengths of pollen tubes have been checked. The tests have been carried out at the drawing of pollen and then 6 months and 12 months after. The pollen viability lowers during this period but it does not disappear. The germination power decreases in Pinus canariensis and P. pinaster, while in Pinus brutia and P. halepensis it is increased by low temperature. Therefore the cryopreservation has to be considered an important method for the maintenance of the germplasm of those plants, like the pines, which are important from the naturalistic point of view.
花粉在室温下的活力会降低，并在几天内消失；相反，冷冻保存可延长花粉的活力。为了检查活力的变化，将地中海松、加那利松、阿勒颇松、海岸松的花粉在-20℃下保存了一年。通过荧光素二乙酸酯测试活力；观察了体外培养96小时的发芽情况，并检查了每日发芽花粉粒的百分比和花粉管的最大长度。测试在花粉采集时进行，然后在6个月和12个月后进行。在此期间花粉活力降低但并未消失。加那利松和海岸松的发芽能力下降，而地中海松和阿勒颇松的发芽能力因低温而提高。因此，对于像松树这样从自然角度来看很重要的植物，冷冻保存必须被视为一种维护其种质的重要方法。
