Halablab M A, Bazin M, Richards L, Pacy J
Division of Biosphere Sciences, King's College London, U.K.
FEMS Microbiol Immunol. 1990 Dec;2(5-6):295-301. doi: 10.1111/j.1574-6968.1990.tb03532.x.
Legionella pneumophila (LP) strains of differing virulence were incubated with a solution of nitroblue-tetrazolium (NBT) at a concentration of 1 mg.ml-1 in the presence of Acanthamoeba polyphaga or human polymorphonuclear neutrophils (PMN). Reduction of NBT to formazan occurred at a faster rate in the presence of virulent strains. Reduction appeared to be temperature dependent; at 37 degrees C the reaction rate was higher than at 20 degrees C. On microscopic examination, deposits of formazan around Legionella cells were observed inside amoebae similar to those deposited in human neutrophils. Electron microscopy revealed electron-dense particles surrounding virulent legionellae, which appeared to be associated with formazan formation. Formazan formation inside amoebae may suggest the presence of a respiratory burst against LP, which is more intense with virulent strains.
将不同毒力的嗜肺军团菌（LP）菌株与浓度为1mg/ml的硝基蓝四氮唑（NBT）溶液在多食棘阿米巴或人多形核中性粒细胞（PMN）存在的情况下进行孵育。在有毒力菌株存在的情况下，NBT还原为甲臜的速度更快。还原似乎与温度有关；在37℃时反应速率高于20℃。显微镜检查发现，在阿米巴内军团菌细胞周围有甲臜沉积物，类似于在人中性粒细胞中沉积的情况。电子显微镜显示，有毒力的军团菌周围有电子致密颗粒，这似乎与甲臜形成有关。阿米巴内甲臜的形成可能表明存在针对LP的呼吸爆发，且对有毒力菌株更为强烈。
