Department of Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8575, Japan.
J Hepatobiliary Pancreat Sci. 2010 Nov;17(6):855-64. doi: 10.1007/s00534-010-0276-2. Epub 2010 Apr 14.
BACKGROUND/PURPOSE: Platelets develop tissue repair and promote liver regeneration. We investigated whether platelets prevented acute liver damage after extended hepatectomy in pigs.
Thrombocytosis was induced by the following two methods; afterwards 80% hepatectomy was performed in pigs. In the first method, the pigs received administration of thrombopoietin [TPO (+) group], and they were compared with a control group [TPO (-) group]. In the second method, the pigs received a splenectomy [Sp (+) group], and theywere compared with another control group [Sp (-) group]. Platelet counts, biochemical examination of blood, and histopathological findings of the residual liver were examined.
Serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and total bilirubin (T-Bil) levels were significantly decreased in the thrombocytotic groups compared with the control groups in the early period after hepatectomy. In the histopathological findings, hemorrhagic necrosis with a bile plug was observed in the control groups, but this phenomenon was not observed in the thrombocytotic groups. On transmission electron microscopy, the sinusoidal endothelial lining was destroyed and detached into the sinusoidal space with enlargement of Disse's spaces in the thrombocytotic groups, but these findings were not observed in the control groups.
An increased number of platelets prevents acute liver damage after extended hepatectomy.
背景/目的：血小板具有组织修复和促进肝脏再生的作用。本研究旨在探讨血小板是否可以预防猪扩大肝切除术后的急性肝损伤。
采用两种方法诱导血小板增多症；然后对猪进行 80%肝切除术。第一种方法是给予促血小板生成素[TPO (+) 组]，并与对照组[TPO (-) 组]进行比较。第二种方法是进行脾切除术[Sp (+) 组]，并与另一个对照组[Sp (-) 组]进行比较。检查血小板计数、血液生化检查和剩余肝脏的组织病理学发现。
与对照组相比，血小板增多症组在肝切除术后早期血清天门冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和总胆红素（T-Bil）水平显著降低。在组织病理学发现中，对照组观察到出血性坏死伴胆栓，但血小板增多症组未观察到这种现象。在透射电子显微镜下，血小板增多症组的窦内皮细胞被破坏并脱落到窦状间隙中，Disse 间隙扩大，但对照组未观察到这种现象。
血小板数量增加可预防扩大肝切除术后的急性肝损伤。
