Center for Biomolecular Interactions Bremen, University of Bremen, Bremen, Germany.
Neurochem Res. 2010 Nov;35(11):1848-56. doi: 10.1007/s11064-010-0251-y. Epub 2010 Aug 24.
High concentrations of 2-deoxy-D-ribose (2dRib) have been reported to cause oxidative stress and to disturb the glutathione (GSH) metabolism of various cell types. Exposure of astrocyte-rich primary cultures to millimolar concentrations of 2dRib or its stereoisomer 2-deoxy-L-ribose, but not the incubation with ribose, 2-deoxyglucose, glucose, fructose or saccharose, lowered the cellular GSH content in a time and concentration dependent manner. After exposure for 4 h to 30 mM 2dRib the cells contained 2dRib in a concentration of about 24 mM. Under these conditions 2dRib did not compromise cell viability and the ability of the cells to synthesise GSH, nor were the cellular ratio of glutathione disulfide (GSSG) to GSH and the extracellular concentrations of GSH or GSSG increased. These data demonstrate that 2dRib deprives viable cultured astrocytes of GSH and suggest that a cellular reaction of GSH with 2dRib or its metabolites is involved in the deprivation of astrocytic GSH.
高浓度的 2-脱氧-D-核糖（2dRib）已被报道会引起氧化应激，并扰乱各种细胞类型的谷胱甘肽（GSH）代谢。暴露于富含星形胶质细胞的原代培养物于毫摩尔浓度的 2dRib 或其立体异构体 2-脱氧-L-核糖，但不是与核糖、2-脱氧葡萄糖、葡萄糖、果糖或蔗糖孵育，以时间和浓度依赖的方式降低细胞内 GSH 含量。在暴露于 30 mM 2dRib 4 小时后，细胞中含有约 24 mM 的 2dRib。在这些条件下，2dRib 不会损害细胞活力和细胞合成 GSH 的能力，也不会增加细胞内谷胱甘肽二硫化物（GSSG）与 GSH 的比值以及细胞外 GSH 或 GSSG 的浓度。这些数据表明，2dRib 剥夺了存活的培养星形胶质细胞中的 GSH，并表明 GSH 与 2dRib 或其代谢物的细胞反应参与了星形胶质细胞 GSH 的剥夺。
