Day Robert C
Department of Biochemistry, University of Otago, Dunedin, Otago, New Zealand.
Methods Mol Biol. 2010;655:321-46. doi: 10.1007/978-1-60761-765-5_22.
High-resolution cellular analysis will help answer many important questions in plant biology including how genetic information is differentially used to enable the formation and development of the plant body. By comparing transcriptome data from distinct cell types during various stages of development, insight can be obtained into the transcriptional networks that underpin the attributes and contributions of particular cells and tissues. Laser microdissection (LM) is a technique that enables researchers to obtain specific cells or tissues from histological samples in a manner conducive to downstream molecular analysis. LM has become an established strategy in many areas of biology and it has recently been adapted for use with many types of plant tissue.
高分辨率细胞分析将有助于解答植物生物学中的许多重要问题，包括遗传信息如何被差异利用以促成植物体的形成和发育。通过比较发育各个阶段不同细胞类型的转录组数据，可以深入了解支撑特定细胞和组织特性及作用的转录网络。激光显微切割（LM）是一种使研究人员能够从组织学样本中获取特定细胞或组织的技术，其方式有利于下游分子分析。激光显微切割已成为生物学许多领域的既定策略，并且最近已适用于多种类型的植物组织。
