Kontoghiorghes G J, May A
Royal Free Hospital School of Medicine, London, UK.
Biol Met. 1990;3(3-4):183-7. doi: 10.1007/BF01140577.
Iron chelators of different physicochemical properties were studied for their ability to donate iron in vitro to uninduced K562 cells, human bone marrow cells and purified human erythroblasts. To a large extent uptake was found to be related to lipophilicity and those chelators able to deliver iron to the cells in significant amounts were also able to deliver iron to ferritin and haem. Some differences in the distribution of iron delivered was observed but no chelator showed exclusive delivery to or rejection of a particular cellular iron compartment. Several chelators could probably substitute for transferrin and be used to probe metabolic events subsequent to iron removal from transferrin. Two chelators which were excellent iron donors were also found to cause considerable inhibition of iron incorporation into haem from transferrin. The implications of this for in vivo toxicity are briefly discussed.
研究了具有不同物理化学性质的铁螯合剂在体外向未诱导的K562细胞、人骨髓细胞和纯化的人成红细胞供铁的能力。在很大程度上，摄取与亲脂性有关，并且那些能够向细胞大量输送铁的螯合剂也能够将铁输送到铁蛋白和血红素中。观察到所输送铁的分布存在一些差异，但没有螯合剂表现出对特定细胞铁区室的排他性输送或排斥。几种螯合剂可能可以替代转铁蛋白，并用于探测铁从转铁蛋白中去除后的代谢事件。还发现两种极好的铁供体螯合剂会显著抑制铁从转铁蛋白掺入血红素中。简要讨论了这对体内毒性的影响。
