用于铁动力学研究的59铁标记转铁蛋白的制备。
The preparation of 59 Fe-labelled transferrin for ferrokinetic studies.
作者信息
Cavill I
出版信息
J Clin Pathol. 1971 Jul;24(5):472-4. doi: 10.1136/jcp.24.5.472.
Abstract
Serum labelled with (59)Fe for ferrokinetic studies should contain only [(59)Fe] transferrin. [(59)Fe] ferric citrate was incubated at 37 degrees C for up to 24 hours with sera having an excess latent iron-binding capacity. The results showed that unbound [(59)Fe] ferric citrate may be present for several hours. An anion exchange resin (IRA 400 Cl(-)) column removed all the [(59)Fe] ferric citrate and left only [(59)Fe] transferrin in the eluate. It is suggested that all (59)Fe-labelled preparations for ferrokinetic studies should be passed through a resin column before injection.
摘要
用于铁动力学研究的用（59）铁标记的血清应仅含[（59）铁]转铁蛋白。将[（59）铁]柠檬酸铁与具有过量潜在铁结合能力的血清在37℃孵育长达24小时。结果显示未结合的[（59）铁]柠檬酸铁可能存在数小时。阴离子交换树脂（IRA 400 Cl（-））柱可除去所有的[（59）铁]柠檬酸铁，洗脱液中仅留下[（59）铁]转铁蛋白。建议所有用于铁动力学研究的（59）铁标记制剂在注射前应通过树脂柱。
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