Chernenkov A Iu, Ivanova S V, Koval'tsova S V, Gracheva L M, Peshekhonov V T, Fedorova I V, Korolev V G
Genetika. 2010 Jun;46(6):742-9.
Gene HSM3 encodes the Hsm3 protein involved in the minor branch in the system responsible for the correction of mismatched bases in DNA structure and controls replicative and reparative spontaneous mutagenesis in yeast Saccharomyces cerevisiae. Spontaneous and UV-induced mutagenesis was studied in three mutant alleles of gene HSM3, and repair effectivity of artificial heteroduplexes was assessed in DNA molecule. The resuts of these studies allowed establishment of the protein domain structure of protein Hsm3 and functions of each domain: the N-terminal domain is responsible for binding to mispaired bases, and the C-terminal domain ensures the interaction with other proteins involved in the system of mismatched base correction.
基因HSM3编码参与DNA结构错配碱基校正系统中次要分支的Hsm3蛋白，并控制酿酒酵母中的复制性和修复性自发诱变。对基因HSM3的三个突变等位基因进行了自发诱变和紫外线诱导诱变研究，并在DNA分子中评估了人工异源双链体的修复效率。这些研究结果有助于确定Hsm3蛋白的结构域结构以及每个结构域的功能：N端结构域负责与错配碱基结合，C端结构域确保与错配碱基校正系统中涉及的其他蛋白质相互作用。
