Karlov G I, Fesenko I A, Andreeva G N, Khrustaleva L I
Genetika. 2010 Jun;46(6):769-73.
Ty1-copia-like retrotransposons were discovered in all studied plants and form a considerable fraction of their genomes. PCR amplification of a pool of fragments of the reverse transcriptase gene of Ty1-copia-like retrotransposons of the tomato genome was conducted and their physical organization in mitotic and meiotic chromosomes was studied. The method of fluorescence in situ hybridization revealed the location of retrotransposons in the pericentromeric heterochromatin and their absence in the nucleolus organizer region. Knowledge of physical distribution of retrotransposons in the tomato genome is important when constructing molecular markers on their basis. Comparative analysis of chromosomal location of retrotransposons in different plant species extends our knowledge of the plant genome evolution.
在所有已研究的植物中都发现了类Ty1-copia逆转座子，它们在植物基因组中占相当大的比例。对番茄基因组中类Ty1-copia逆转座子逆转录酶基因片段池进行了PCR扩增，并研究了它们在有丝分裂和减数分裂染色体中的物理组织。荧光原位杂交方法揭示了逆转座子在着丝粒周围异染色质中的位置，以及它们在核仁组织区的缺失。了解逆转座子在番茄基因组中的物理分布对于基于它们构建分子标记很重要。对不同植物物种中逆转座子染色体定位的比较分析扩展了我们对植物基因组进化的认识。
