Department of Biology, University "Tor Vergata," Rome, Italy.
Cancer Biother Radiopharm. 2010 Aug;25(4):497-503. doi: 10.1089/cbr.2010.0787.
Melanoma is one of the most aggressive forms of tumor, being responsible for about 80% of skin cancer deaths. Much effort is being directed at obtaining less-toxic anticancer therapies, and the combination between low cytotoxic doses of chemotherapeutic drugs and natural differentiative compounds seems to be of particular importance. The present study was undertaken to examine the possible role of a combination therapy using paclitaxel (PTX) as chemotherapeutic molecule and theophylline (TH) as differentiative agent in the prevention of metastasis in B16-F10 melanoma-bearing C57BL6/N mice. In vitro proliferation studies demonstrated that TH enhanced the antiproliferative effect of PTX. In the in vivo experiments, a highly sensitive computerized image analysis method, performed on histological lung sections of mice injected with melanoma cells, was used to quantify the efficacy of the treatments. This study demonstrated that the simultaneous treatment of mice with TH and a low dose of PTX produced a similar anti-invasive effect than that caused by highly toxic PTX concentration.
黑色素瘤是最具侵袭性的肿瘤之一，约占皮肤癌死亡人数的 80%。目前正在努力寻找毒性较低的抗癌疗法，而低细胞毒性化疗药物与天然分化化合物的联合似乎具有特别重要的意义。本研究旨在探讨紫杉醇（PTX）作为化疗分子和茶碱（TH）作为分化剂联合治疗在预防 B16-F10 黑色素瘤荷瘤 C57BL6/N 小鼠转移中的可能作用。体外增殖研究表明，TH 增强了 PTX 的抗增殖作用。在体内实验中，对注射黑色素瘤细胞的小鼠的组织学肺切片进行了高度敏感的计算机图像分析，以量化治疗效果。这项研究表明，同时用 TH 和低剂量 PTX 治疗小鼠可产生与高毒性 PTX 浓度相似的抗侵袭作用。
