Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 912, Taiwan, Republic of China.
J Fish Biol. 2009 Feb;74(2):357-76. doi: 10.1111/j.1095-8649.2008.02049.x.
Morphometric and genetic data were used to compare two sympatric and morphologically similar species, Acanthopagrus berda and Acanthopagrus taiwanensis, in Dapeng Bay, South-western Taiwan. A principle component analysis of morphological data indicated a distinction between the two species, with pectoral fin length and eye diameter accounting for 32.27% of the variation. Interspecific sequence divergence, based on mtDNA cytochrome b (0.118 +/- 0.01), was larger than intraspecific divergences between haplotypes (0.007 for A. taiwanensis and 0.003 for A. berda). Individuals of the two species clustered into different groups in three phylogenetic trees with 100% bootstrap support. The mean observed heterozygosity for eight microsatellite loci was 0.471 +/- 0.202 for A. taiwanensis and 0.637 +/- 0.145 for A. berda. Nei's unbiased measure of interspecific genetic distance (D(S)) was 1.334. F(ST) (0.134) and R(ST) (0.404) values indicated significant differentiation between species. An unrooted neighbour-joining tree was constructed by allele-sharing distances and the factorial correspondence analysis split all specimens into two distinct clusters. The results of morphometric, mtDNA and microsatellite analyses indicated the presence of two species, A. taiwanensis and A. berda.
形态和遗传数据被用于比较台湾西南部大鹏湾的两种同域且形态相似的物种，赤鳍石斑鱼和台湾石斑鱼。形态数据分析的主成分分析表明这两个物种存在区别，胸鳍长度和眼睛直径占变异的 32.27%。基于线粒体 DNA 细胞色素 b 的种间序列分歧（0.118 +/- 0.01）大于种内单倍型间的分歧（0.007 为台湾石斑鱼和 0.003 为赤鳍石斑鱼）。个体在三个基于 100% bootstrap 支持的系统发育树中聚集成不同的组。8 个微卫星位点的平均观察杂合度分别为台湾石斑鱼的 0.471 +/- 0.202 和赤鳍石斑鱼的 0.637 +/- 0.145。种间遗传距离（D(S)）的 Nei 无偏估计值为 1.334。F(ST)（0.134）和 R(ST)（0.404）值表明物种间存在显著分化。基于等位基因共享距离构建了无根邻接树，因子对应分析将所有标本分为两个不同的聚类。形态、mtDNA 和微卫星分析的结果表明存在两种物种，即台湾石斑鱼和赤鳍石斑鱼。
