Division of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
J Fish Biol. 2009 May;74(7):1487-98. doi: 10.1111/j.1095-8649.2009.02216.x.
The biochemical and stress responses of two Indian major carps, rohu Labeo rohita and mrigal Cirrhinus mrigala were studied after acclimating them to four preset temperatures (26, 31, 33 and 36 degrees C) for 30 days. The blood glucose and liver glycogen levels showed an inverse trend in both the species and were significantly different in L. rohita at higher temperatures. The decrease in the liver glycogen level of C. mrigala, however, was not significant. Plasma cortisol levels increased significantly whereas the ascorbic acid content in the brain and kidney of both the species decreased significantly with increasing temperatures. Total lipid content in the liver of both the species decreased significantly with increasing acclimation temperatures. The phospholipid concentration decreased in L. rohita with increasing acclimation temperatures, and in C. mrigala the values decreased up to 33 degrees C and increased at 36 degrees C. In C. mrigala, the cholesterol level decreased up to 33 degrees C and then increased at 36 degrees C, but the absolute value was lower in comparison to L. rohita. The cholesterol levels, however, were not significantly different in L. rohita. Triglycerides and free fatty acids concentrations decreased significantly with increasing acclimation temperatures in both the species. The present study indicates species-specific metabolic responses of L. rohita and C. mrigala to thermal acclimation.
在将两种印度主要鲤鱼，罗非鱼（Labeo rohita）和鲤鱼（Cirrhinus mrigala）适应四种预设温度（26、31、33 和 36°C）30 天后，研究了它们的生化和应激反应。两种鱼类的血糖和肝糖原水平呈相反趋势，在较高温度下罗非鱼的血糖和肝糖原水平显著不同。然而，鲤鱼的肝糖原水平下降并不显著。血浆皮质醇水平显著升高，而两种鱼类大脑和肾脏中的抗坏血酸含量随着温度的升高而显著下降。两种鱼类的肝总脂质含量随着适应温度的升高而显著下降。罗非鱼的磷脂浓度随着适应温度的升高而降低，而鲤鱼的数值在 33°C 时降低，在 36°C 时升高。在鲤鱼中，胆固醇水平在 33°C 时降低，然后在 36°C 时升高，但绝对值低于罗非鱼。然而，罗非鱼的胆固醇水平没有显著差异。两种鱼类的甘油三酯和游离脂肪酸浓度随着适应温度的升高而显著下降。本研究表明，罗非鱼和鲤鱼对热适应的代谢反应具有种特异性。
