Ahammad MM, Bhattacharyya D, Jana BB
Department of Zoology, University of Kalyani, Kalyani-, West Bengal, 741235, India.
Cryobiology. 1998 Dec;37(4):318-24. doi: 10.1006/cryo.1998.2127.
Rohu (Labeo rohita), catla (Catla catla) and mrigal (Cirrhinus mrigala) embryos were examined for hatching success after short-term (8-13 h) storage at low temperature (4 degreesC) using three concentrations of three cryoprotectants, methanol (1, 2, and 3 M), dimethyl sulfoxide (1.282, 1.923, and 2.564 M, equal to 10, 15, and 20%), and glycerol (1.087, 1.63, and 2.174 M, equal to 10, 15, and 20%), separately as well as together with a single concentration of sucrose (0.5 M). The aim was to determine the toxicity of cryoprotectants for fish embryos at 4 degreesC. Of the cryoprotectants used, methanol was found to be most suitable for low-temperature storage in the refrigerator. The hatching rate of embryos was significantly higher when sucrose was added to methanol when compared with methanol alone. Of the three concentrations of methanol tested, survival was maximal at 2 M in the presence of 0.5 M sucrose (rohu, 57.5 +/- 5.24; catla, 47.5 +/- 5.24; and mrigal, 32. 5 +/- 5.24). Both rohu and catla embryos survived with either 1 or 2 M methanol, with or without the addition of sucrose, but the addition of sucrose was essential for survival of mrigal at 1 and 2 M methanol. Copyright 1998 Academic Press.
使用三种浓度的三种冷冻保护剂，分别为甲醇（1M、2M和3M）、二甲基亚砜（1.282M、1.923M和2.564M，分别相当于10%、15%和20%）以及甘油（1.087M、1.63M和2.174M，分别相当于10%、15%和20%），并将其与单一浓度的蔗糖（0.5M）一起使用，研究了印度鲤（Labeo rohita）、印度鲃（Catla catla）和墨头鱼（Cirrhinus mrigala）胚胎在低温（4摄氏度）下短期（8 - 13小时）保存后的孵化成功率。目的是确定4摄氏度下冷冻保护剂对鱼类胚胎的毒性。在所使用的冷冻保护剂中，发现甲醇最适合在冰箱中进行低温保存。与单独使用甲醇相比，当向甲醇中添加蔗糖时，胚胎的孵化率显著更高。在测试的三种甲醇浓度中，在0.5M蔗糖存在下，2M甲醇时的存活率最高（印度鲤，57.5 +/- 5.24；印度鲃，47.5 +/- 5.24；墨头鱼，32.5 +/- 5.24）。印度鲤和印度鲃胚胎在有或没有添加蔗糖的情况下，使用1M或2M甲醇均可存活，但对于1M和2M甲醇浓度下的墨头鱼，添加蔗糖对其存活至关重要。版权所有1998年学术出版社。
