Laboratory of Animal Diversity and Systematics, Katholieke Universiteit Leuven, Ch. Deberiotstraat 32, B-3000 Leuven, Belgium.
J Fish Biol. 2009 Jun;74(9):2047-68. doi: 10.1111/j.1095-8649.2009.02294.x.
The fine scale morphological and genetic dynamics of successive waves of Anguilla anguilla glass eel recruitment was studied over a 2 year period at a southern European Mediterranean location (Camargue, France) with continuous recruitment. Using morphometric [total length (L(T)), mass (M), condition (K) and pigmentation stage] as well as genetic (allozyme) markers, the aim was to test for the existence of temporally separated spawning groups and explore the relation between genetic variability and morphological heterogeneity of recruits. The results showed that L(T), M and K varied over time, being highest from the end of summer to winter (peaking in December) and lowest in spring (lowest in April). The pigmentation stages within monthly samples were highly diverse with a heterogeneous seasonal pattern. Allozyme data showed high genetic variability values within samples, but low genetic differentiation among samples (F(ST) = 0.003, P < 0.05). Pairwise comparisons between samples indicated a positive correlation between genetic differentiation and difference in recruitment time (days), with a marked increase in genetic differentiation around 250 days between monthly recruitment samples. Furthermore, genetic diversity increased with the number of pigmentation stages per sample and was negatively correlated with the North Atlantic Oscillation (NAO) index during the putative year of trans-oceanic migration. No correlation, however, was found between the level of multilocus heterozygosity (MLH) and growth variables. A situation of genetic patchiness with fluctuating parental contribution can thus best explain the patterns observed, although the existence of two separate spawning periods cannot be excluded. More discriminatory and sensitive genetic markers, such as (neutral and adaptive) microsatellites, could probably provide additional insights into the most probable hypothesis explaining the population structure and recruitment heterogeneity of A. anguilla.
连续几批玻璃鳗补充的精细规模形态和遗传动态在一个具有连续补充的南欧地中海地区（法国卡马格）进行了为期 2 年的研究。使用形态计量学[全长（L（T））、质量（M）、条件（K）和色素阶段]和遗传（同工酶）标记物，目的是测试是否存在时间上分离的产卵群体，并探讨遗传变异性与补充群体形态异质性之间的关系。结果表明，L（T）、M 和 K 随时间变化，从夏末到冬季最高（12 月达到峰值），春季最低（4 月最低）。每月样本中的色素阶段高度多样化，具有异质的季节性模式。同工酶数据显示样本内遗传变异性值高，但样本间遗传分化低（F（ST）= 0.003，P < 0.05）。样本间的成对比较表明，遗传分化与补充时间（天）之间存在正相关，每月补充样本之间的遗传分化在 250 天左右明显增加。此外，遗传多样性随着每个样本中的色素阶段数量增加而增加，与北大西洋涛动（NAO）指数呈负相关，而在推测的跨洋迁徙年期间。然而，多基因座杂合度（MLH）水平与生长变量之间没有相关性。因此，波动的亲代贡献的遗传斑块状可以最好地解释所观察到的模式，尽管不能排除存在两个独立的产卵期。更具区分性和敏感性的遗传标记，如（中性和适应性）微卫星，可能会为解释 A. anguilla 种群结构和补充异质性的最可能假设提供更多的见解。
