Chen Qiong, Liu Lihong, Cao Hong
Department of Pathology, Chengdu No.2 Pepole's Hospital, Chengdu, Sichuan 610017, China.
Zhongguo Fei Ai Za Zhi. 2008 Aug 20;11(4):559-62. doi: 10.3779/j.issn.1009-3419.2008.04.029.
It was reported that telomerase was associated with the oncogenesis and progression of cancer, and to be the common targets of cancer therapy. The mechanism of matrine on lung cancer in vitro is not clear. We studied the effect of matrine on growth of human lung adenocarcinoma A549 cells and the mechanism related with telomerase.
MTT was used for measuring A549 cells viability, Hoechst 33342-propidium iodide fluorescent staining for observing apoptotic cells, flow cytometry (FCM) for analyzing cell cycle and apoptosis, and immunocytochemistry for measuring the protein expressions of c-myc and hTERT in A549 cells.
Matrine inhibited the proliferation of A549 cells with a time-dose-dependent manner (P <0.05). Hoechst 33342-propidium iodide staining showed apoptotic cells with chromatin condensation and fragmentation of nuclei. FCM analysis indicated elevating rate of cells in G0/G1 phase, lowering rate of that in S phase and the highering apoptotic rate. The levels of c-myc and hTERT protein expression in the matrine group was lower than that in the control group (P <0.05), and AOD of c-myc showed positive correlation with AOD of hTERT (r =0.633, P <0.01).
The inhibitory effect of matrine on A549 cells may be related to the lower expression of c-myc and hTERT.
据报道，端粒酶与癌症的发生和发展相关，并且是癌症治疗的常见靶点。苦参碱对肺癌的体外作用机制尚不清楚。我们研究了苦参碱对人肺腺癌A549细胞生长的影响及其与端粒酶相关的机制。
采用MTT法检测A549细胞活力，用Hoechst 33342-碘化丙啶荧光染色观察凋亡细胞，流式细胞术（FCM）分析细胞周期和凋亡情况，免疫细胞化学法检测A549细胞中c-myc和hTERT的蛋白表达。
苦参碱以时间-剂量依赖方式抑制A549细胞增殖（P<0.05）。Hoechst 33342-碘化丙啶染色显示有染色质浓缩和核碎裂的凋亡细胞。FCM分析表明G0/G1期细胞比例升高，S期细胞比例降低，凋亡率升高。苦参碱组c-myc和hTERT蛋白表达水平低于对照组（P<0.05），c-myc的吸光度与hTERT的吸光度呈正相关（r=0.633，P<0.0l）。
苦参碱对A549细胞的抑制作用可能与c-myc和hTERT表达降低有关。
