Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, 23071 Jaén, Spain.
Cardiovasc Res. 2011 Jan 1;89(1):98-108. doi: 10.1093/cvr/cvq264. Epub 2010 Aug 24.
non-coding RNA has been recently demonstrated to be a novel mechanism for modulation of gene expression at the post-transcriptional level. The importance of microRNAs in the cardiovascular system is now apparent. Mutations of distinct microRNAs have provided evidence for fundamental roles of microRNAs during cardiovascular development. However, there is limited information about global microRNA profiles during mouse heart development. In this study, we have gained insight from the expression profiles of microRNAs during mouse ventricular development by microarray and qRT-PCR analysis.
our microarray analysis reveals that relatively few microRNAs display either increasing or decreasing expression profiles during ventricular chamber formation. Interestingly, most of the differentially expressed microRNAs display a rather discrete peak of expression at particular developmental stages. Furthermore, we demonstrate that microRNA-27b (miR-27b) displays an overt myocardial expression during heart development and that the transcription factor-encoding gene Mef2c is an miR-27b target.
our data present a comprehensive profile of microRNA expression during ventricular maturation, providing an entry point for investigation of the functional roles of the most abundantly and differentially expressed microRNAs during cardiogenesis.
非编码 RNA 最近被证明是一种在转录后水平调节基因表达的新机制。microRNAs 在心血管系统中的重要性现在已经很明显。不同 microRNAs 的突变为 microRNAs 在心血管发育过程中的基本作用提供了证据。然而，关于小鼠心脏发育过程中全局 microRNA 谱的信息有限。在这项研究中，我们通过 microarray 和 qRT-PCR 分析深入了解了 microRNAs 在小鼠心室发育过程中的表达谱。
我们的 microarray 分析表明，在心室腔形成过程中，相对较少的 microRNAs 表现出增加或减少的表达谱。有趣的是，大多数差异表达的 microRNAs 在特定发育阶段显示出相当离散的表达峰值。此外，我们证明了 microRNA-27b（miR-27b）在心脏发育过程中表现出明显的心肌表达，并且转录因子编码基因 Mef2c 是 miR-27b 的靶标。
我们的数据提供了心室成熟过程中 microRNA 表达的综合图谱，为研究在心脏发生过程中最丰富和差异表达的 microRNAs 的功能作用提供了切入点。
