Urrutia R A, Rivolta C M, Valentich M A, Monis B
Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Cell Mol Biol. 1990;36(5):547-55.
Microspectrophotometric DNA measurements in exocrine pancreas of essential fatty acid-deficient (EFAD) and EFA-sufficient (EFAS) rats which received a single intraperitoneal injection of the carcinogen nitrosomethylurea (NMU) or saline (SAL) was the subject of the present report. The DNA content of acinar pancreatic cells of SAL-injected EFAD and EFAS rats was diploid. NMU-induced pancreatic focal acinar cell hyperplasia (FACH) had one main cell population with a diploid content, whereas in the intervening parenchyma there were diploid and tetraploid cells. The number of tetraploid cells was smaller in EFAD rat pancreas than in EFAS indicating a diet dependent effect. NMU-induced FACH had a diploid distribution pattern indicating that cells are in a G1, quiescent phase, contrasting with AZA-induced similar lesions which showed an abnormal ploidy. It remains to be established whether DNA phenotypic traits of NMU and AZA induced FACH reflect the neoplastic potentials of both types of lesions. The decreased number of tetraploid cells in EFAD rat pancreas is in keeping with data indicating a promoting effect of the EFA linoleic and arachidonic acids on growth rate of certain cell populations in vitro.
本报告的主题是对必需脂肪酸缺乏（EFAD）和必需脂肪酸充足（EFAS）的大鼠外分泌胰腺进行微分光光度法DNA测量，这些大鼠接受了单次腹腔注射致癌物亚硝基甲基脲（NMU）或生理盐水（SAL）。注射生理盐水的EFAD和EFAS大鼠胰腺腺泡细胞的DNA含量为二倍体。NMU诱导的胰腺局灶性腺泡细胞增生（FACH）有一个主要细胞群，其含量为二倍体，而在其间的实质中有二倍体和四倍体细胞。EFAD大鼠胰腺中四倍体细胞的数量比EFAS大鼠少，表明存在饮食依赖性效应。NMU诱导的FACH具有二倍体分布模式，表明细胞处于G1静止期，这与氮杂丝氨酸诱导的类似病变形成对比，后者显示出异常的倍性。NMU和氮杂丝氨酸诱导的FACH的DNA表型特征是否反映了这两种病变的肿瘤发生潜能，仍有待确定。EFAD大鼠胰腺中四倍体细胞数量的减少与表明必需脂肪酸亚油酸和花生四烯酸对某些细胞群体体外生长速率有促进作用的数据一致。
